Article Figures & Data
Figures
- FIGURE 1.
Structures of 11C-M-MPEP, 11C-M-FPEP, 18F-FE-MPEP, 18F-FPEP, and 11C-ABP688.
- FIGURE 2.
Radiosynthesis of 11C-ABP688.
- FIGURE 3.
(A) Typical saturation curve of 11C-ABP688 binding to rat brain membrane. (B) Representative Scatchard plot of 11C-ABP688 binding. Results from 3 independent determinations gave a KD of 1.7 ± 0.2 nmol/L and a Bmax of 231 ± 18 fmol/mg of protein.
- FIGURE 4.
(A) Autoradiographic illustration of 20-μm unwashed horizontal (approximately −4.10 mm from Bregma and 5.90 mm interaural (24) slice of rat brain 8 min after intravenous injection of 11C-ABP688. (B and C) Series of horizontal slices of 11C-ABP688 distribution in wt-mouse (B) and ko-mouse (C).
- FIGURE 5.
11C-ABP688 uptake in some brain regions of rat and in blood. Animals were sacrificed 30 min after injection. Control group (n = 3) received polyethylene glycol:H2O (1:1) and 11C-ABP688, and test group (n = 3) received coinjection of M-MPEP (1.0 mg/kg of body weight) and 11C-ABP688. Data are expressed as percentage normalized injected dose (%ID norm) per gram of tissue ± SD.
- FIGURE 6.
Biodistribution of 11C-ABP688 in some brain regions of wt-mice (n = 4), mGluR5-ko-mice (n = 4), and blood. Animals were sacrificed 20 min after injection, and data are expressed as percentage of normalized injected dose (%ID norm) per gram of tissue ± SD.
- FIGURE 7.
Representative horizontal images of 11C-ABP688 uptake in head of 2 rats. Control animal (A and B) received 29.4 MBq (injected mass, 1.3 nmol) of radiotracer, and test animal (C and D) received coinjection of M-MPEP (1.0 mg/kg of body weight) and 11C-ABP688 (5.37 MBq, injected mass, 1.7 nmol). Images were normalized to injected radioactivity and body weight. Data were reconstructed with bin size of 0.3 mm from 0 to 30 min after injection. ce = cerebellum; cp = caudate putamen; hi = hippocampus; lg = lacrimal gland. In B and D, whole brain and specific regions are bordered with white (24).
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