OtherBasic Science Investigations

Monitoring Left Ventricular Dilation in Mice with PET

Lars Stegger, Klaus P. Schäfers, Ulrich Flögel, Lefteris Livieratos, Sven Hermann, Christoph Jacoby, Petra Keul, Edward M. Conway, Otmar Schober, Jürgen Schrader, Bodo Levkau and Michael Schäfers
Journal of Nuclear Medicine September 2005, 46 (9) 1516-1521;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    PET and MR images depict a short-axis slice through the heart of a wild-type mouse (A) with normal heart size and a transgenic mouse (B) with dilated left ventricle. MR images shown were acquired in end-diastole.

  • FIGURE 2.
    FIGURE 2.

    Midmyocardial (solid line) and endo- or epicardial (dashed lines) contours superimposed on 18F-FDG PET short-axis image (A) and vertical long-axis image (B) of mouse heart as measured by PET. Only the midmyocardial contour is used in this study.

  • FIGURE 3.
    FIGURE 3.

    Comparison of LV volume indices LV-VIPET/MRI (in μL) measured by PET and MRI. Equal fractional contributions of end-diastolic or end-systolic volumes were used for LV-VIMRI (xED = xES = 0.5). (A) Scatter plot of LV volume indices measured by PET vs. MRI. Confidence curves for regression fit as well as confidence curves of individual values are also shown (dashed lines, α = 0.05). (B) Difference of PET and MRI measurements plotted against the mean of both measurements. Horizontal lines for mean (solid) and mean ± 2 SD (dashed) are included. Values for linear fit (intercept, 30.0; slope, 0.011) are not shown.

Tables

  • TABLE 1

    Weight and Sex of Wild-Type and Transgenic Mice (Matched Pairs)

    AgeWild-type (g)Transgenic (g)Sex
    Young21.624.3F
    27.427.9M
    25.927.3M
    19.516.8F
    23.023.6F
    φ23.5φ24.0
    Old34.032.3M
    26.826.9F
    35.632.3M
    28.132.7M
    29.722.3M
    φ30.8φ29.3

    • ↵φ indicates mean weight.

  • TABLE 2

    Choice of Fractional Contributions of End-Diastolic and End-Systolic Volumes in Calculation of LV-VIMRI and its Influence on Correlation Between PET- and MRI-Derived LV-VI

    Fraction xED of end-diast. volume in LV-VIMRIFraction xES of end-syst. volume in LV-VIMRICorrelation coefficient (R)Intercept of correlation lineSlope of correlation line
    0.01.00.8811.00.83
    0.20.80.89−0.260.85
    0.50.50.89−16.90.88
    0.80.20.89−32.60.89
    1.00.00.88−41.90.89

    • end-diast. = end-diastolic; end-syst. = end-systolic.

  • TABLE 3

    Matrix of Group Results

    PETYoungOldP value
    Wild-type65.0 ± 16.982.6 ± 20.90.185 (NS)
    Transgenic73.8 ± 15.0129.3 ± 15.3<0.001 (S)
    P value0.413 (NS)0.004 (S)
    MRIYoung*†Old*†P value*†
    Wild-type94.7 ± 14.6106.2 ± 12.30.214 (NS)
    111.3 ± 16.0†122.8 ± 12.3†0.27† (NS)
    Transgenic114.7 ± 15.2160.5 ± 25.70.012 (S)
    132.3 ± 15.8†174.6 ± 25.5†0.014† (S)
    P value0.067 (NS)0.006 (S)
    0.071† (NS)0.004† (S)

    • ↵* First row of results for equal fractional contributions of end-diastolic or end-systolic volumes in definition of LV-VIMRI (xED = xES = 0.5).

    • ↵† Denotes values with unequal fractional contributions (xED = 0.8, xES = 0.2).

    • NS = nonsignificant results; S = significant results.

      Mean values of midmyocardial volumes (in μL) ± SDs for all 4 groups of animals (young—old, wild-type—transgenic) are presented together with t-test results (P values). Results are presented separately for PET and MRI measurement techniques.

Back to top

In this issue

Print
Download PDF
Article Alerts
Email Article
Citation Tools
Bookmark this article

Jump to section