Article Figures & Data
Figures
- FIGURE 1.
Radio-FPLC of rat serum 10 min after incubation with processed 45Ti shows 45Ti bound to a single protein (A) and FPLC of apotransferrin (B). mAU = milliabsorbance units.
- FIGURE 2.
Radio-FPLC of 45Ti-transferrin. mAU = milliabsorbance units.
- FIGURE 3.
Radio-FPLC of serum from BALB/c mouse injected with 45Ti-transferrin, 4 h after injection. mAU = milliabsorbance units.
- FIGURE 4.
Two coronal microPET image slices (A and C) and 1 transaxial slice (B) of female BALB/c mouse bearing EMT-6 tumors (denoted by arrows) in both legs (right-hand side: 1 large tumor; left-hand side: 2 small tumors) injected with 27.72 MBq (750 μCi) 45Ti-transferrin, 1 h 40 min after injection.
- FIGURE 5.
Coronal (A), transaxial (B), and sagittal (C) image slices of female BALB/c mouse bearing EMT-6 murine mammary carcinoma tumor (denoted by arrow) in nape of the neck injected with 7.59 MBq (205 μCi) 45Ti-citrate, 2 h after injection.
- FIGURE 6.
Schematic of iron (A) vs. titanium (B) (postulated) binding configurations.
Tables
Tissue %ID/g ± SD 10 min 1 h Blood 0.166 ± 0.032 0.145 ± 0.022 Lung 0.261 ± 0.031 0.192 ± 0.043 Liver 4.609 ± 0.870 4.700 ± 0.431 Spleen 7.274 ± 0.250 7.011 ± 3.150 Kidney 0.049 ± 0.009 0.051 ± 0.004 Muscle 0.003 ± 0.0004 0.004 ± 0.001 Fat 0.002 ± 0.001 0.003 ± 0.0005 n = 3 for each time point.
Tissue %ID/g ± SD 10 min 2 h 4 h 7 h Blood 5.85 ± 0.43 3.94 ± 0.66 2.01 ± 1.03 1.59 ± 0.37 Lung 2.39 ± 0.82 1.70 ± 0.40 1.17 ± 0.40 0.87 ± 0.24 Liver 1.55 ± 0.15 1.52 ± 0.17 1.35 ± 0.37 1.41 ± 0.10 Spleen 1.15 ± 0.18 0.95 ± 0.18 0.76 ± 0.23 0.74 ± 0.13 Kidney 1.41 ± 0.25 1.60 ± 0.21 1.39 ± 0.34 1.41 ± 0.15 Muscle 0.10 ± 0.03 0.18 ± 0.01 0.26 ± 0.05 0.26 ± 0.03 Fat 0.09 ± 0.02 0.18 ± 0.09 0.21 ± 0.05 0.23 ± 0.03 Heart 1.11 ± 0.11 1.09 ± 0.05 0.73 ± 0.21 0.56 ± 0.12 Brain 0.23 ± 0.03 0.17 ± 0.10 0.08 ± 0.04 0.10 ± 0.03 Bone (whole) 0.52 ± 0.10 1.09 ± 0.02 1.50 ± 0.20 1.97 ± 0.24 n = 4 for each time point.
Tissue %ID/g ± SD 10 min 2 h 4 h 7 h Blood 2.35 ± 1.22 1.38 ± 0.51 0.86 ± 0.74 0.64 ± 0.17 Lung 1.10 ± 0.48 0.64 ± 0.24 0.46 ± 0.28 0.34 ± 0.12 Liver 0.46 ± 0.25 0.56 ± 0.23 0.59 ± 0.34 0.61 ± 0.29 Spleen 0.41 ± 0.20 0.38 ± 0.18 0.31 ± 0.14 0.311 ± 0.16 Kidney 0.70 ± 0.25 0.56 ± 0.09 0.73 ± 0.26 0.66 ± 0.07 Muscle 0.22 ± 0.004 0.25 ± 0.03 0.21 ± 0.07 0.13 ± 0.02 Fat 0.13 ± 0.05 0.17 ± 0.01 0.13 ± 0.02 0.09 ± 0.01 Heart 0.79 ± 0.35 0.44 ± 0.10 0.27 ± 0.17 0.23 ± 0.06 Brain 0.09 ± 0.03 0.06 ± 0.04 0.04 ± 0.03 0.04 ± 0.01 Bone (whole) 0.67 ± 0.13 1.80 ± 0.13 3.02 ± 0.51 3.71 ± 0.04 n = 4 for each time point.
- TABLE 4
Biodistribution of 45Ti-Transferrin in BALB/c Mice Bearing EMT-6 Murine Mammary Carcinoma Tumors
Tissue %ID/g ± SD 2 h 4 h 24 h Blood 37.2 ± 2.45 32.3 ± 3.34 10.2 ± 1.03 Lung 13.2 ± 2.42 13.4 ± 0.50 Liver 14.5 ± 1.57 17.3 ± 1.02 12.8 ± 0.58 Spleen 8.65 ± 1.71 11.0 ± 0.64 Kidney 12.1 ± 1.61 11.8 ± 1.34 Gallbladder 3.43 ± 2.25 3.08 ± 2.23 Muscle 3.20 ± 0.29 4.28 ± 0.70 3.50 ± 0.37 Fat 3.93 ± 1.89 4.78 ± 0.70 Heart 8.99 ± 0.84 9.51 ± 1.04 Brain 1.13 ± 0.15 0.75 ± 0.04 Bone (whole) 10.5 ± 0.63 15.2 ± 0.74 Tumor 8.57 ± 2.04 14.6 ± 1.83 14.9 ± 1.74 n = 4 for each time point.
- TABLE 5
Biodistribution of 67Ga-Citrate in BALB/c Mice Bearing EMT-6 Murine Mammary Carcinoma Tumors
Tissue %ID/g ± SD 2 h 4 h 24 h Blood 15.8 ± 0.96 14.4 ± 0.68 2.77 ± 0.77 Lung 7.22 ± 0.50 7.27 ± 0.36 Liver 5.71 ± 0.47 7.75 ± 0.41 7.31 ± 0.55 Spleen 4.77 ± 0.82 6.32 ± 0.72 Kidney 5.97 ± 0.73 7.02 ± 0.75 Gallbladder 2.88 ± 1.33 2.31 ± 0.89 Muscle 2.97 ± 0.47 2.78 ± 0.15 1.08 ± 0.16 Fat 2.35 ± 0.57 2.22 ± 0.23 Heart 4.25 ± 0.75 4.21 ± 0.34 Brain 0.52 ± 0.10 0.47 ± 0.08 Bone (whole) 10.3 ± 1.69 13.4 ± 0.86 Tumor 7.05 ± 1.40 8.72 ± 0.98 7.26 ± 0.69 n = 4 for each time point.
