Article Figures & Data
Figures
- FIGURE 1.
First experiment. (A) Bar graph showing mean loss of body weight (+SEM) for mice treated 1, 3, 5, and 8 d with 4% (w/v) DSS in their drinking water. Weight loss after 5 and 8 d of DSS administration is significant (P < 0.01). (B) Bar graph showing mean increase in wet colon weight (+SEM) for mice treated 1, 3, 5, and 8 d with 4% (w/v) DSS in their drinking water. Weight increase after 8 d of DSS administration is significant (P < 0.05).
- FIGURE 2.
First experiment. (A) Bar graph showing histologic score of colon resection specimens (+SEM) for mice treated 1, 3, 5, and 8 d with 4% (w/v) DSS in their drinking water. Increase after 5 and 8 d of DSS administration is significant (P < 0.01). (B) Bar graph showing increase in scintigraphic uptake ratio (+SEM) for mice treated 1, 3, 5, and 8 d with 4% (w/v) DSS in their drinking water. Increase in colon uptake ratio of neutrophils after 8 d of DSS administration is significant (P < 0.05).
- FIGURE 3.
First experiment. Images are representative transverse pinhole SPECT slices of abdomen of mice treated for 1 d (A), 3 d (B), 5 d (C), and 8 d (D) with 4% (w/v) DSS in their drinking water. Increasing uptake of 99mTc-HMPAO–labeled neutrophils is seen in colon (arrows). Physiologic uptake of activity is seen in bone marrow of pelvis (p), spine (s), or limbs (l). Activity in urinary bladder (D) has been masked.
- FIGURE 4.
Second experiment. Images are representative transverse pinhole SPECT slices of abdomen of mice treated for 8 d with 4% (w/v) DSS and injected with labeled neutrophils incubated with control antibody (A) or with aCD97 (B). Significant decrease in uptake of 99mTc-HMPAO–labeled neutrophils is seen in colon of mice with aCD97-blocked neutrophil migration (arrow, B). Physiologic uptake of activity is seen in bone marrow of pelvis (p) or limbs (l).
- FIGURE 5.
Second experiment. Bar graph shows mean scintigraphic uptake ratio (+SEM) of labeled neutrophils in mice treated for 8 d with 4% (w/v) DSS in their drinking water. Uptake is significantly lower (P < 0.01) after blocking of neutrophil migration with aCD97 antibody than after aspecific control antibody.
Tables
Group DSS (d) Weight loss (g) Colon weight (g/cm, ×100) Histologic score Scintigraphic uptake ratio A 1 0.00 ± 0.07 1.045 ± 0.005 0.37 ± 0.13 1.11 ± 0.02 B 3 0.08 ± 0.11 1.053 ± 0.046 3.25 ± 0.52* 1.21 ± 0.17 C 5 1.17 ± 0.11* 1.170 ± 0.105 8.13 ± 1.23* 1.96 ± 0.21 D 8 1.98 ± 0.62* 1.993 ± 0.137* 17.17 ± 0.60* 5.40 ± 1.58* ↵* P < 0.05.
