OtherBasic Science Investigations

Discrepant Recovery Course of Sympathetic Neuronal Function and β-Adrenoceptors in Rat Hearts After Reperfusion Following Transient Ischemia

Bun-ichi Kato, Takashi Nozawa, Norio Igarashi, Makoto Nonomura, Nozomu Fujii, Akihiko Igawa, Hidetsugu Asanoi, Yoshihisa Yamada, Minoru Inoue and Hiroshi Inoue
Journal of Nuclear Medicine June 2004, 45 (6) 1074-1080;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Schematic illustrations of cyanopindolol.

  • FIGURE 2.
    FIGURE 2.

    Schematic diagram for autoradiographic quantification of myocardial accumulation of 131I-MIBG and 125I-ICYP. Two regions of interest were determined.

  • FIGURE 3.
    FIGURE 3.

    Levels of plasma (A) and myocardial (B) NE and myocardial NE ratio of IR to RR (C) in rats with transient coronary occlusion. (A) Hatched bars indicate sham-operated rats (n = 6, 5, and 6 on days 1, 14, and 28, respectively). Black bars indicate rats with coronary occlusion (n = 4, 6, and 7 on days 1, 14, and 28, respectively). (B) Hatched bars indicate sham-operated rats (n = 8, 6, and 6 on days 1, 14, and 28, respectively). White bars and black bars indicate IR and RR, respectively, in rats with coronary occlusion (n = 7, 9, and 7 on days 1, 14, and 28, respectively). Data are mean ± SD. **P < 0.05 vs. coronary occlusion rats on day 1; #P < 0.05 vs. sham-operated rats on day 1; †P < 0.01 vs. RR on each experimental day; *P < 0.01 vs. IR on day 1; ‡P < 0.01 vs. on day 1.

  • FIGURE 4.
    FIGURE 4.

    Representative examples of dual-tracer autoradiography with 131I-MIBG (top) and 125I-ICYP (bottom) on days 1, 14, and 28 after reperfusion following 15-min ischemia in rat hearts. Images were obtained from transverse section of hearts at level of papillary muscle. 125I-ICYP images were derived from same slices that produced 131I-MIBG images on each day.

  • FIGURE 5.
    FIGURE 5.

    (A) Myocardial 131I-MIBG accumulation of IR (white bars) and RR (black bars) (n = 7, 8, 7, 7, and 8 on days 1, 3, 7, 14, and 28, respectively). (B) 131I-MIBG accumulation ratio of IR to RR. Data are mean ± SD. †P < 0.01 vs. IR on day 1; ‡P < 0.01 vs. IR on day 14; **P < 0.05 vs. RR on day 1; *P < 0.05 vs. RR on day 14; #P < 0.05 vs. on day 1.

  • FIGURE 6.
    FIGURE 6.

    (A) Myocardial 125I-ICYP accumulation of IR (white bars) and RR (black bars). Number of rats is same as in Figure 5. (B) 125I-ICYP accumulation ratio of IR to RR. Data are mean ± SD. †P < 0.05 vs. IR on day 28; #P < 0.05 vs. on day 28.

Tables

  • TABLE 1

    Hemodynamic Data

    ParameterDay 1Day 14Day 28
    I/R (n = 6)SH (n = 8)I/R (n = 7)SH (n = 6)I/R (n = 6)SH (n = 7)
    HR (bpm)442 ± 31416 ± 44398 ± 36393 ± 37399 ± 51371 ± 29
    LVSP (mm Hg)111 ± 8117 ± 6111 ± 8117 ± 9120 ± 6112 ± 12
    dP/dtmax (×103 mm Hg/s)9.4 ± 1.111.5 ± 1.310.2 ± 1.611.0 ± 2.410.7 ± 1.610.4 ± 2.4
    −dP/dtmin (×103 mm Hg/s)6.4 ± 0.97.6 ± 1.06.0 ± 1.07.2 ± 0.96.4 ± 1.06.5 ± 1.4

    • I/R = coronary occlusion-reperfusion rats; n = number of rats; SH = sham-operated rats; HR = heart rate in beats per minute; LVSP = LV systolic pressure; dP/dtmax and −dP/dtmin = maximum and minimum values of rate of change in LV pressure.

    • Data are presented as mean ± SD.

  • TABLE 2

    β-Receptor Density and Kd in Membrane Preparations

    ParameterDay 3 (n = 7)
    IRRR
    Bmax (fmol/mg)83 ± 17*100 ± 12
    Kd (nmol/L)0.18 ± 0.050.17 ± 0.03

    • ↵* P < 0.05 vs. RR.

    • n = number of rats.

    • Data are presented as mean ± SD.

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