OtherBasic Science Investigations

Characterization of 18F-FDG Uptake in Human Endothelial Cells In Vitro

Simone Maschauer, Olaf Prante, Markus Hoffmann, J. Thiess Deichen and Torsten Kuwert
Journal of Nuclear Medicine March 2004, 45 (3) 455-460;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Radio-TLC of intracellular metabolites of 18F-FDG in HUVECs. Radio-TLC was performed on silica gel–coated plastic sheets with acetonitrile/tetrabutylammonium hydroxide, 9.5 mmol/L, 8:2 (v/v), as eluant. 18F-FDG-1,6-dP = 18F-FDG-1,6-diphosphate; 18F-FDG-6P = 18F-FDG-6-phosphate.

  • FIGURE 2.
    FIGURE 2.

    Influence of phlorizin on 18F-FDG uptake by HUVECs. Values are mean ± SD of a single experiment performed in triplicate (n = 3). One similar experiment yielded qualitatively identical results. c = concentration.

  • FIGURE 3.
    FIGURE 3.

    Inhibition of 18F-FDG uptake in HUVECs by cytochalasin B. Cytochalasin B competitively inhibits 18F-FDG uptake with an IC50 of 0.16 μmol/L. Values are mean ± COV (% of mean) of 2 independent experiments performed in triplicate (n = 6). c = concentration.

  • FIGURE 4.
    FIGURE 4.

    Influence of deprivation of glucose and sodium depletion on 18F-FDG uptake in HUVECs. Glucose deprivation significantly enhances 18F-FDG uptake by a factor of 2.7 (P < 0.02). Values are mean ± SD of a single experiment performed in triplicate (n = 3). Two similar experiments yielded qualitatively identical results. t = time.

  • FIGURE 5.
    FIGURE 5.

    Immunocytochemical staining of HUVECs. Monoclonal anti-VEGF receptor-2 (KDR) was used as the primary antibody. (A) Negative control. (B) Primary antibody.

  • FIGURE 6.
    FIGURE 6.

    Influence of VEGF stimulation on 18F-FDG uptake in HUVECs. HUVECs from the fourth passage were treated with VEGF (50 ng/mL, 2 h) and incubated with 18F-FDG for 3 h at 4°C in PBS. Values are mean ± SD of 2 independent experiments (n = 6; P < 0.003).

  • FIGURE 7.
    FIGURE 7.

    18F-FDG uptake in HUVECs, GLIOs, and human monocytes differentiated to macrophages (HMMs). HUVECs were from the fourth passage, HMMs differentiated to macrophages during 14 d of culture, and GLIOs were incubated for 3 h with 18F-FDG at 4°C in PBS. Values are mean ± SD of multiple independent experiments: HUVECs, n = 22; GLIOs, n = 6; HMMs, n = 6.

Tables

  • TABLE 1

    Influence of Unlabeled Glucose Concentration in Incubation Buffer on 18F-FDG Uptake in HUVECs

    Glucose concentration (mmol/L)18F-FDG uptake (%/mg)
    059.1 ± 8.8
    0.252.9 ± 4.0
    220.8 ± 8.4
    2012.2 ± 8.5
    2008.9 ± 5.9

    • Uptake values are mean ± SD of 2 independent experiments (n = 5) obtained for HUVECs from the fourth passage (3-h incubation at 4°C in PBS).

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