OtherBasic Science Investigations

3-O-Methyl-6-18F-Fluoro-l-Dopa, a New Tumor Imaging Agent: Investigation of Transport Mechanism In Vitro

Ralf Bergmann, Jens Pietzsch, Frank Fuechtner, Beate Pawelke, Bettina Beuthien-Baumann, Bernd Johannsen and Joerg Kotzerke
Journal of Nuclear Medicine December 2004, 45 (12) 2116-2122;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Reaction scheme of synthesis of OMFD (9).

  • FIGURE 2.
    FIGURE 2.

    Representative curves showing time course of OMFD uptake in HT-29 cells without (control) and with various concentrations of BCH in incubation medium (A) and OMFD uptake velocity (k) in HT-29 cells in presence of various concentrations of BCH (B). Data are mean ± SEM (n = 4).

  • FIGURE 3.
    FIGURE 3.

    Influence of specific amino acid transporter inhibitors, sodium, and choline (without sodium) on OMFD uptake in HT-29, FaDu, and RBE4 cells. Data are mean ± SEM (n = 4). **P < 0.01; ***P < 0.001 (Student t test, SPSS software package).

  • FIGURE 4.
    FIGURE 4.

    Kinetics of OMFD release in HT-29 cells in presence and absence of BCH at 37°C after preloading of OMFD. Data are mean ± SEM (n = 4).

  • FIGURE 5.
    FIGURE 5.

    Concentration-dependent OMFD uptake in adenocarcinoma cells (HT-29) (A) and squamous carcinoma cells (FaDu) (B). OMFD uptake was measured with varying concentrations of unlabeled 19F-OMFD between 0 and 150 μmol/L. Data are mean ± SEM (n = 4). (C and D) Corresponding Eadie–Hofstee plots. v = initial velocity of OMFD uptake; S = substrate concentration.

  • FIGURE 6.
    FIGURE 6.

    Comparison of OMFD and 3H-l-leucine uptake in adenocarcinoma cells (HT-29) in absence and presence of various BCH concentrations after incubation at 1, 2, 3, 5, 7, and 10 min. Data are mean ± SEM (n = 4).

  • FIGURE 7.
    FIGURE 7.

    (A) Influence of incubation temperature on OMFD uptake in adenocarcinoma (HT-29) without (control) and with 6 mmol BCH per liter. (B) Influence of various concentrations of sodium azide on OMFD uptake in adenocarcinoma (HT-29) and squamous carcinoma (FaDu) cells. Data are mean ± SEM (n = 4).

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