Article Figures & Data
Figures
- FIGURE 1.
Mice were injected with approximately 740 kBq 125I-annexin V labeled using IODO-BEADS. (Top) Mice were given KI in drinking water for 3 d before injection. (Bottom) Mice received no added iodide in diet.
- FIGURE 2.
(A) Activity incorporated in annexin V increases with increasing pH. (B) Activity of annexin V, as measured by its ability to bind to PS, is unaffected by pH of labeling reaction. Error bars represent 95% confidence intervals, based on 3 independent experiments.
- FIGURE 3.
Bolton-Hunter-labeled annexin V binds to apoptotic cells only in presence of Ca2+. Binding is abolished by addition of unlabeled annexin at 100-fold excess. Data are from 2 independent experiments.
- FIGURE 4.
Biologically active fraction of Bolton-Hunter-iodinated annexin V can be purified by affinity chromatography. Labeled annexin was loaded onto column in calcium-containing buffer; this buffer was used to elute unbound activity (fractions 1–3). Column was washed with buffer containing EDTA to elute bound activity (fractions 4–8).
- FIGURE 5.
(A) 125I-Annexin V binding in 4 untreated animals (black bars) compared with irradiated animals (hatched bars). Annexin V uptake is expressed relative to 131I-BSA uptake. Error bars represent 95% confidence intervals. (B) TUNEL staining of spleen sections: (Top) control; (Bottom) irradiated.
- FIGURE 6.
Mice injected with Bolton-Hunter-labeled 125I-annexin V. (A and B) Mice were injected with 925 kBq 125I-annexin V and imaged 2 h later. (A) Control animals. (B) Mice were injected with anti-FAS antibody. (C) Mice received 370 kBq 125I-annexin V and were imaged 24 h after injection.
