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Rapid Imaging of Experimental Colitis with 99mTc-Interleukin-8 in Rabbits

Stefan Gratz, Huub J.J.M. Rennen, Otto C. Boerman, Wim J.G. Oyen and Frans H.M. Corstens
Journal of Nuclear Medicine June 2001, 42 (6) 917-923;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Scintigraphic images of rabbits with experimental colitis, imaged at 2 min and at 1, 2, and 4 h after injection, together with separate image of dissected colon of 99mTc-HMPAO-granulocytes (A). In vivo, no nonspecific bowel uptake is seen until 4 h after injection and 99mTc-HYNIC-IL-8 (B).

  • FIGURE 2.
    FIGURE 2.

    CIs of both radiotracers at 1 h (A) and 4 h (B) postinjection (p.i.) compared with severity of macroscopic inflammation (grade 0 = normal; grade1 = inflammation; grade 2 = ulceration). Error bars represent SEM.

  • FIGURE 3.
    FIGURE 3.

    Macroscopic aspect of colonic mucosa in TNBS-induced acute colitis. Scoring of inflammation is shown: 0 = normal (colon descending); 1 = inflammation (sigmoid colon); 2 = ulceration (rectum).

Tables

  • TABLE 1

    Uptake of 99mTc-HYNIC-IL-8 and 99mTc-HMPAO-Granulocytes in Affected and Nonaffected Colonic Segments

    Time99mTc-HYNIC-IL-899mTc-HMPAO-granulocytesP†
    Nonaffected* (%ID)Affected (%ID)Nonaffected* (%ID)Affected (%ID)
    2 min—0.012 ± 0.003—0.010 ± 0.003<0.7
    1 h—0.034 ± 0.005—0.029 ± 0.003<0.5
    2 h—0.07 ± 0.017—0.021 ± 0.002<0.03
    4 h—0.08 ± 0.02—0.019 ± 0.006<0.03

    • ↵* No visual uptake.

    • ↵† Mann–Whitney test: 99mTc-HYNIC-IL-8 vs. 99mTc-HMPAO-granulocytes. Level of significance was set at P < 0.05.

    • Colonic uptake (%ID [mean ± SEM]) of both tracers is shown at different time points.

  • TABLE 2

    Biodistribution of 99mTc-HYNIC-IL-8 and 99mTc-HMPAO-Granulocytes in 2 Groups of Rabbits with Experimental Colitis

    Parameter4 h after injectionP*
    99mTc-HYNIC-IL-8 (%ID/g)99mTc-HMPAO-granulocytes (%ID/g)
    Blood0.044 ± 0.0010.30 ± 0.03<0.007
    Small intestine0.032 ± 0.0030.024 ± 0.003<0.06
    Nonaffected colon  tissue0.025 ± 0.0040.023 ± 0.008<0.70
    Affected colon tissue0.41 ± 0.040.091 ± 0.055<0.007
    Feces
        Nonaffected colon0.005 ± 0.0010.015 ± 0.003<0.35
        Affected colon0.013 ± 0.0020.022 ± 0.007<0.26
    Lung0.27 ± 0.090.18 ± 0.01<0.70
    Spleen0.67 ± 0.220.83 ± 0.12<0.15
    Kidney1.86 ± 0.280.20 ± 0.01<0.007
    Liver0.091 ± 0.0050.21 ± 0.03<0.007

    • ↵* Mann–Whitney test: 99mTc-HYNIC-IL-8 vs. 99mTc-HMPAO-granulocytes. Level of significance was set at P < 0.05.

    • Biodistribution of 2 groups of rabbits (5 rabbits per group) is shown at 4 h after injection. Data are expressed as mean ± SEM.

  • TABLE 3

    Relative Uptake of 99mTc-HYNIC-IL-8 and 99mTc-HMPAO-Granulocytes in Stool and Colonic Wall and Absolute Uptake of 99mTc-HYNIC-IL-8 and 99mTc-HMPAO-Granulocytes for Ratio of Inflamed Healthy Colon

    Parameter4 h after injectionP*
    99mTc-HYNIC-IL-899mTc-HMPAO-granulocytes
    %IDRelative uptake%IDRelative uptake
    Stool in inflamed colon1.07(12.6 ± 3.5)0.38(19.8 ± 3.5)<0.42
    Inflamed colonic wall5.18(87.3 ± 3.5)1.70(80.1 ± 3.5)<0.51
    %ID/g%ID/g
    Ratio of inflamed to healthy colon18.5 ± 4.14.1 ± 1.4<0.007

    • ↵* For 99mTc-HYNIC-IL-8 vs. 99mTc-HMPAO-granulocytes. Data are expressed as mean ± SEM. Level of significance was set at P < 0.05.

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