Article Figures & Data
Figures
- FIGURE 1.
(A) %ID/g* of 131I delivered intravenously via β-d-IAZGP in various tissues at various times after administration (n = 3). Symbols represent R3327-AT tumor (•), liver (▴), kidney (□), blood (○), spleen (▪), lungs (>), muscle (▵), and brain (t). At least 90% of the marker is renally excreted over first 5–6 h followed by much slower hepatobiliary excretion. (B) T/B (○) and T/M (▵) ratios of tissue-specific activities from data in A. Maximum sensitivity of this marker technique is expressed after 6 h.
- FIGURE 2.
Frequency distribution of median Po2 values obtained from 41 R3327-AT tumors.
- FIGURE 3.
Median Po2 of individual R3327-AT tumors versus specific activity of tumor β-d-IAZGP (○) and [99mTc]HL-91 (•) measured 6 h after administration (n = 26).
- FIGURE 4.
%ID/g* [99mTc]HL-91 versus %ID/g* β-d-IAZGP in individual tumors 6 h after administration.
Tables
- TABLE 1.
Effect of Various Anesthetic Procedures on Eppendorf Microelectrode Measurements Obtained from Individual Dunning R3327-AT Tumors*
Animal no. (gender) Tumor weight (g) Restrained Anesthetized with halothane and Ketamine-xylazine O2 Carbogen Air A B A B A B A B A B 1 (F) 10.7 35.4 19.8 0 24.7 0 18.3 0 3.2 59.4 2 (F) 3.0 59.4 14.2 0 24.5 0 18.8 0 4.0 50.8 3 (F) 6–10† 2.0 82.3 18.3 0.8 13.7 0 15.3 0 1.1 89.1 4 (F) 4.3 51 12.8 0 14.4 0 13.1 0 5 (F) 7.0 37.2 21.9 0 19.1 0 22 0 6 (F) 2.4 70.2 25.2 0 28.8 0 26.3 0 4.7 56.0 7 (F) 4.2 7.6 42.1 9.0 16.7 5.0 49.0 10.5 15.9 8 (F) 5.7 20.9 0 20.3 0 22.4 0 9.9 13.7 6.7 29.4 9 (F) 7.7 3.5 85.0 13.3 12.9 5.4 41.2 4.1 70.6 6.8 14.7 10 (F) 7.1 2.0 85.0 11.8 2.0 6.6 10.8 9.2 22.5 6.2 5.9 11 (F) 4.8 3.7 86.6 13.1 0 10.4 4.0 12.9 0 4.5 54.9 12 (M) 4.4 13.7 2.7 17.5 0 8.2 0 37.8 0 7.6 3.6 13 (M) 6.6 13.6 0 7.6 21.6 12.2 17.6 10.7 12.5 6.2 23.5 14 (F) 3.1 7.2 32.2 32.2 4.2 4.3 58.8 19.3 8.5 6.1 18.8 15 (M) 5.6 10.9 0 21.0 3.5 19.1 0 33.7 0 4.6 60.8 Mean 6.5 7.5 44.6 17.2 4.1 14.6 12.1 16.7 9.6 5.1 38.9 ±SE 0.4 1.4 8.6 1.7 1.8 2.1 5.3 2.6 4.8 0.5 7.7 Increase from restrained value 13/15 11/15 12/15 5/12 Decrease from restrained value 2/15 4/15 3/15 7/12 Mean‡ 4.3 63.1 17.2 3.7 13.2 3.2 15.2 11.8 4.8 41.5 ±SE 0.7 6.8 2.3 1.9 2.7 1.1 2.2 7.0 0.7 11.2 Increase from restrained value 10/10 8/10 10/10 5/7 Decrease from restrained value 0/10 2/10 0/10 2/7 * A, median Po2 in mm Hg (reading varied from 99 to 125); B, percentage of measurements <5 mm Hg.
↵† These tumor weights were estimated from caliper measurements. Tumors in animals 7 through 15 were excised and weighed at end of each experiment.
↵‡ Means from those tumors whose median Po2 values under conditions of restraint were <10 mm Hg.
- TABLE 2.
Measurements of Median Po2 by Eppendorf Microelectrodes and Retention of Hypoxic Markers, Gb-d-IAZGP and [99mTc]HL-91, in Same Dunning R3327-AT Tumors 6 Hours After Administration to Tumor-Bearing Animals
Rat no. Tumor weight (g) Median Po2 (mm Hg) Gb-d-IAZGP [99mTc]HL-91 %ID/g* T/B T/M %ID/g* T/B T/M 1 9.7 2.9 (114*) 0.057 3.3 3.9 0.091 1.5 5.0 2 8.0 2.9 (84) 0.067 3.5 4.9 0.089 1.7 6.8 3 8.9 3.8 (92) 0.082 3.6 4.0 0.134 1.7 6.8 4 9.2 8.9 (105) 0.054 2.5 4.5 0.080 1.3 4.9 5 9.2 5.2 (76) 0.070 3.4 4.1 0.108 1.5 5.5 6 8.4 4.6 (105) 0.063 2.9 3.5 0.138 1.6 7.1 7 5.2 6.2 (88) 0.072 3.4 4.8 0.131 1.6 7.0 8 5.4 6.5 (102) 0.079 3.8 4.8 0.125 1.6 7.1 9 6.0 7.1 (101) 0.074 3.4 3.9 0.135 1.6 7.1 10 7.9 2.8 (104) 0.082 3.5 4.3 0.149 1.7 7.0 11 5.2 4.6 (104) 0.083 3.4 3.6 0.147 1.7 7.4 12 11.8 4.9 (97) 0.078 3.4 4.6 0.109 1.6 6.0 13 6.0 3.7 (106) 0.071 3.2 4.5 0.105 1.5 6.4 14 7.6 3.2 (103) 0.063 2.8 3.6 0.077 1.2 5.4 15 8.0 6.7 (100) 0.075 4.2 5.0 0.109 1.9 7.3 16 7.3 15.4 (119) 0.038 3.0 5.2 0.106 1.7 7.1 17 8.5 4.6 (111) 0.086 4.6 5.4 0.095 2.0 7.6 18 8.1 2.9 (115) 0.071 3.9 5.0 0.098 1.5 5.9 19 6.9 4.7 (85) 0.075 4.4 5.5 0.110 1.9 7.6 20 4.6 3.6 (105) 0.084 3.6 3.9 0.146 1.8 7.1 21 6.0 6.8 (121) 0.056 2.9 3.1 0.091 1.2 5.3 22 13.1 4.1 (102) 0.073 3.3 3.9 0.106 1.6 5.6 23 5.2 3.6 (99) 0.064 3.9 4.3 0.100 1.7 6.9 24 5.6 3.7 (92) 0.067 3.2 3.8 0.107 1.5 7.2 25 7.3 3.5 (109) 0.076 3.1 3.5 0.095 1.5 6.7 26 12.8 4.5 (105) 0.067 3.2 3.2 0.106 1.7 5.6 Mean 7.8 5.1 (102) 0.070 3.4 4.3 0.111 1.6 6.5 SE 0.45 0.51 (2.1) 0.002 0.09 0.13 0.004 0.04 0.17 ↵* Number of spatially distinct measurements of tumor Po2 used to define the median value.
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