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OtherBASIC SCIENCE INVESTIGATIONS

Serial Changes in 14C-Deoxyglucose and 201Tl Uptake in Autoimmune Myocarditis in Rats

Naoki Tokita, Shinji Hasegawa, Eiichiro Tsujimura, Kenji Yutani, Tohru Izumi and Tsunehiko Nishimura
Journal of Nuclear Medicine February 2001, 42 (2) 285-291;
Naoki Tokita
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Shinji Hasegawa
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Eiichiro Tsujimura
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Kenji Yutani
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Tohru Izumi
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Tsunehiko Nishimura
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  • FIGURE 1.
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    FIGURE 1.

    Pathologic grading of extent of inflammatory damage. Mild damage (A), moderate damage (B), severe damage (C), and normal control (D). (×200).

  • FIGURE 2.
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    FIGURE 2.

    Total cardiac uptakes of DG (A) and 201Tl (B) in acute, subacute, and chronic phases of myocarditis. Open and closed bars indicate average values of all phases in controls and myocarditis, respectively.

  • FIGURE 3.
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    FIGURE 3.

    Dual-tracer autoradiograms of myocardium in acute (A and B) and chronic (C and D) phases of autoimmune myocarditis. Arrowheads denote sites of severe infiltration corresponding to areas of high GLUT1 expression. DG image (A and C) and 201Tl image (B and D).

  • FIGURE 4.
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    FIGURE 4.

    Microscopic findings in acute (A–C) and chronic (D–F) phases of myocarditis. Hematoxylin and eosin (H.E.) staining (A and D), GLUT1 (B and E), and GLUT4 (C and F). Arrowheads indicate sites of high DG uptake (×1).

Tables

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    TABLE 1.

    Regional Uptakes of DG and 201T1

    PhaseDG (%)201Tl (%)
    SevereModerateMildControlSevereModerateMildControl
    Acute317.5 ± 103.3*169.6 ± 59.9143.1 ± 107.9100.0 ± 46.448.4 ± 21.5†72.1 ± 34.688.4 ± 31.9100.0 ± 11.4
    Subacute—367.1 ± 175.4*245.7 ± 61.0100.0 ± 12.8—83.7 ± 23.1109.5 ± 27.2100.0 ± 23.0
    Chronic—129.0 ± 43.3124.9 ± 59.8100.0 ± 48.2—76.6 ± 29.6100.2 ± 25.4100.0 ± 7.5
    • ↵* P < 0.0005 vs. control.

    • ↵† P < 0.005 vs. control.

    • View popup
    TABLE 2.

    Visual Evaluation of Regional GLUT1 and GLUT4 Expressions

    PhaseGLUT1 (degree)GLUT4 (degree)
    SevereModerateMildControlSevereModerateMildControl
    Acute2.87 ± 0.30*1.73 ± 0.680.93 ± 0.150.87 ± 0.300.20 ± 0.301.60 ± 0.441.87 ± 0.30†1.07 ± 0.15
    Subacute—1.73 ± 0.680.87 ± 0.300.93 ± 0.15—1.20 ± 0.301.93 ± 0.151.40 ± 0.55
    Chronic—1.00 ± 0.620.87 ± 0.510.93 ± 0.15—1.07 ± 0.761.40 ± 0.281.40 ± 0.55
    • ↵* P < 0.0001 vs. control.

    • ↵† P < 0.0005 vs. control.

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Journal of Nuclear Medicine
Vol. 42, Issue 2
February 1, 2001
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Serial Changes in 14C-Deoxyglucose and 201Tl Uptake in Autoimmune Myocarditis in Rats
Naoki Tokita, Shinji Hasegawa, Eiichiro Tsujimura, Kenji Yutani, Tohru Izumi, Tsunehiko Nishimura
Journal of Nuclear Medicine Feb 2001, 42 (2) 285-291;

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Serial Changes in 14C-Deoxyglucose and 201Tl Uptake in Autoimmune Myocarditis in Rats
Naoki Tokita, Shinji Hasegawa, Eiichiro Tsujimura, Kenji Yutani, Tohru Izumi, Tsunehiko Nishimura
Journal of Nuclear Medicine Feb 2001, 42 (2) 285-291;
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