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Meeting ReportBasic Science

PET/CT Myocardial Inflammation Imaging in Myocarditis Rats by Using a Translocator Protein Tracer 18F-FDPA

Tiantian Mou, Xiaoli Zhang, Tian Yi, Jing Tian and Xiang Li
Journal of Nuclear Medicine August 2022, 63 (supplement 2) 3331;
Tiantian Mou
1Beijing Anzhen Hospital
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Xiaoli Zhang
1Beijing Anzhen Hospital
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Tian Yi
1Beijing Anzhen Hospital
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Jing Tian
1Beijing Anzhen Hospital
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Xiang Li
1Beijing Anzhen Hospital
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Abstract

3331

Introduction: In this study, a translocator protein 18 kDa (TSPO) targeted radiotracer, N,N-diethyl-2-(2-(4-18F-fluorophenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide (18F-FDPA) was evaluated as a potential non-invasive imaging agent of myocarditis.

Methods: 18F-FDPA was radiolabeled automatically by a one-step nucleophilic reaction. Rats in myocarditis group were injected 0.2 mL porcine myosin suspension (1 mg myosin in 0.1 mL buffered aqueous glycerol solution mixed with 0.1 mL Freund's complete adjuvant) into the foot pad at Day 1 and Day 7. On Day 21, normal rats (n=4) and myocarditis rats (n=4) were injected about 30 MBq 18F-FDPA, and PET/CT imaging was underwent (Inveon PET/CT, Siemens, Germany) at 30 min post injection. Global heart uptake of 18F-FDPA in normal and myocarditis rats were analyzed by Inveon Research Workplace. Maximum standardized uptake value (SUVmax) and mean standardized uptake value (SUVmean) were quantitatively obtanined After PET/CT imaging, the hearts were harvested. The hematoxylin and eosin (HE) staining, TSPO and CD68 immunofluorescence staining were performed. The inflammation and normal myocardium region in myocarditis rats were defined by HE staining, respectively. The target to background ratio (TBR) in each rat between inflammation region and normal region was calculated as: TBR = SUVmean in the inflammation region / SUVmean in the normal region.

Results: The total synthesis time of 18F-FDPA was 68 min using CFN-MPS200 module. The non-decay corrected radiochemical yields were 19.8 ± 2.8% (n = 6). The radiochemical purities were ≥ 99%. The molar activities were 140-225 GBq/μmol. The SUVmean (5.12 ± 0.38 vs. 3.18 ±0.09, P<0.001) and SUVmax (8.80 ± 0.82 vs. 6.35 ± 1.08, P=0.011) in myocarditis rats were significantly high than that in normal rats. The HE staining result indicated that the hearts in myocarditis rats were infiltrated with massive inflammatory cells and the TBR of 18F-FDPA in the inflammation region was 1.64 ± 0.16. The immunofluorescence staining in these regions showed positive TSPO expression, as well as positive CD68 expression, indicating the increased myocardium uptake of 18F-FDPA was related with macrophages.

Conclusions: High 18F-FDPA activity was revealed in the myocarditis rats, especially in the myocardial regions infiltrated with macrophages. 18F-FDPA could be a potential cardiac inflammation imaging agent.

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Journal of Nuclear Medicine
Vol. 63, Issue supplement 2
August 1, 2022
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PET/CT Myocardial Inflammation Imaging in Myocarditis Rats by Using a Translocator Protein Tracer 18F-FDPA
Tiantian Mou, Xiaoli Zhang, Tian Yi, Jing Tian, Xiang Li
Journal of Nuclear Medicine Aug 2022, 63 (supplement 2) 3331;

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PET/CT Myocardial Inflammation Imaging in Myocarditis Rats by Using a Translocator Protein Tracer 18F-FDPA
Tiantian Mou, Xiaoli Zhang, Tian Yi, Jing Tian, Xiang Li
Journal of Nuclear Medicine Aug 2022, 63 (supplement 2) 3331;
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