Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • View or Listen to JNM Podcast
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Follow JNM on Twitter
  • Subscribe to our RSS feeds
Meeting ReportNovel Multimodality & Nonradioactive Probes

Nanostructured Polyvinylpyrrolidone-Curcumin Conjugates allowed for Kidney-targeted Treatment of Cisplatin Induced Acute Kidney Injury

Muhsin Younis, Hao Wei, Dawei Jiang, Bo Yu, Todd Barnhart, Jonathan Engle, Xiaoli Lan and Weibo Cai
Journal of Nuclear Medicine August 2022, 63 (supplement 2) 2827;
Muhsin Younis
1University of Wisconsin - Madison
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hao Wei
2Huazhong University of Science and Technology, Tongji Medical College
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dawei Jiang
3Huazhong University of Science and Technology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bo Yu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Todd Barnhart
4University of Wisconsin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Engle
1University of Wisconsin - Madison
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoli Lan
5Wuhan Union Hospital
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Weibo Cai
1University of Wisconsin - Madison
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
Loading

Abstract

2827

Introduction: Acute kidney injury (AKI) has a high mortality rate which can be partially attributed to inefficient renal delivery of therapeutic drugs. Nanoparticles are promising candidates in the development of theranostic agents for AKI since they can be made with controllable kidney targeting properties, such as kidney specificity and size control to make it past glomerular filtration. We developed a series of polyvinylpyrrolidone (PVP)-curcumin nanoparticles (PCurNP) as controllable delivery nanosystems which meet the renal excretion threshold of about 45 kDa for the purpose of treating AKI. They were analyzed for treatment of cisplatin-induced AKI. Since these nanoparticles can be imaged with positron emission tomography (PET), they make up a PET image-guided drug delivery system for the kidney.

Methods: PVP-curcumin nanoparticles were synthesized using PVP of molecular weights 10 kDa (M10), 29 kDa (M29), or 40 kDa (M40), each conjugated to curcumin. Nanoparticle formation was due to the hydrophobic interaction of curcumin inside the conjugates (forming PCurNP M10, PCurNP M29, and PCurNP M40). Cisplatin-induced AKI mouse models were established with intraperitoneal injections of cisplatin. in BALB/c mice with three groups: healthy, AKI, and AKI+treatment (n=3/group). Serum and renal tissue were collected to assess the development and treatment of AKI before treatment. At 2h after cisplatin injection, AKI+treatment mice were intravenously injected with PCurNP M10 and AKI was evaluated using a tubular injury score (TIS). For PET imaging, the nanoparticles were also radiolabeled with 89Zr through mixing, with the oxygen donors of the PVP-curcumin conjugates being capable of chelation. BALB/c mice were injected in the tail vein with 89Zr-PCurNP M10, M29, and M40, after which dynamic PET scans were taken for 30 min (n=3/group). Images were also taken at 1, 2, 3, 5, 14, and 24 h post injection (p.i.). For ex vivo validation of biodistribution results (24 h p.i.), major organs were harvested, weighed, and radioactivity was measured using a gamma counter. In vitro and in vivo biocompatibility studies were performed. Human embryonic kidney cells (HEK 293) were incubated with PCurNP at different concentrations for 24 h then cell viability was analyzed. Healthy male BALB/c mice were injected in the tail vein with PCurNP M10 to analyze the toxicity of these nanoparticles.

Results: In mice with cisplatin-induced AKI, PCurNP M10 were able to restore some kidney function, indicated by a lower TIS than in the control group. A score of either 0 or 1 was observed, where 0 indicates no lesions, 1 indicates swelling of tubular epithelial cells. In PET images, high kidney uptake was seen at all times, in addition to a significant signal in the bladder. At 2.25 min p.i., 89Zr-PCurNP M10 uptake in the kidney was 36.4±13.9 %ID/g, and decreased to 16.7±4.6 %ID/g by 30 min. While M10 had higher kidney uptake than M40, the duration of blood circulation of M40 was higher than of M10. At 24 h p.i., the accumulation of M10 in the kidney was 15.4±0.3 %ID/g, which was about 1.7 times higher than that of M29 (6.3±0.1 %ID/g) and 1.8 times higher than that of M40 (5.7±1.2 %ID/g). Low accumulation was found in the liver (max 22.2±9.1 %ID/g) and spleen (max 14.4±2.2 %ID/g). These results agreed with the ex vivo biodistribution study. In the biocompatibility studies, PCurNPs were not found to be cytotoxic to HEK 293 cells nor to BALB/c mice.

Conclusions: We synthesized a nanoparticle delivery system for the kidney which meets the renal excretion threshold of about 45 kDa in order to treat AKI. Dynamic PET imaging has shown that these nanoparticles rapidly accumulate in the kidney as intended. They are not cytotoxic. The smallest of these nanoparticles, PCurNP M10, has been shown to have therapeutic efficacy against cisplatin-induced AKI. Due to their ability to be readily imaged with dynamic PET, they represent a viable image-guided drug delivery system for the kidney.

Figure
  • Download figure
  • Open in new tab
  • Download powerpoint
Previous
Back to top

In this issue

Journal of Nuclear Medicine
Vol. 63, Issue supplement 2
August 1, 2022
  • Table of Contents
  • Index by author
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Nanostructured Polyvinylpyrrolidone-Curcumin Conjugates allowed for Kidney-targeted Treatment of Cisplatin Induced Acute Kidney Injury
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
Nanostructured Polyvinylpyrrolidone-Curcumin Conjugates allowed for Kidney-targeted Treatment of Cisplatin Induced Acute Kidney Injury
Muhsin Younis, Hao Wei, Dawei Jiang, Bo Yu, Todd Barnhart, Jonathan Engle, Xiaoli Lan, Weibo Cai
Journal of Nuclear Medicine Aug 2022, 63 (supplement 2) 2827;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Nanostructured Polyvinylpyrrolidone-Curcumin Conjugates allowed for Kidney-targeted Treatment of Cisplatin Induced Acute Kidney Injury
Muhsin Younis, Hao Wei, Dawei Jiang, Bo Yu, Todd Barnhart, Jonathan Engle, Xiaoli Lan, Weibo Cai
Journal of Nuclear Medicine Aug 2022, 63 (supplement 2) 2827;
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Dual-modality imaging of peptide M2pep modified nanomicelle targeting tumor associated macrophages
  • Radiochemistry Development of 52Mn for Characterization of PET/MR Imaging Agents
Show more Novel Multimodality & Nonradioactive Probes

Similar Articles

SNMMI

© 2025 SNMMI

Powered by HighWire