Article Figures & Data
Figures
- FIGURE 1.
Heart rate response to 11C-CS1P1 injection: stable heart rate before injection, 5 min after injection, and at discharge, as captured on 12-lead electrocardiogram (P > 0.1).
- FIGURE 2.
Qualitative biodistribution of 11C-CS1P1. (A) Summed activity PET/CT images for representative male and female participants. (B) Decay-corrected activity is high in liver early in scanning session but is clearly concentrated in gallbladder later in scans, compatible with hepatobiliary excretion.
- FIGURE 3.
Qualitative biodistribution in typical participant. Summed activity on 11C-CS1P1 PET/CT images is expressed as SUV. (A–D) Uptake in brain is highest in gray matter and relatively low in white matter. Increased update is seen in salivary glands (B), left ventricle (C), and liver (D). Common bile duct is appreciated (arrowhead). (E) Parenchymal uptake within kidneys is seen but without activity in collecting system and with scant amounts of small-bowel uptake.
- FIGURE 4.
Non–decay-corrected (blue) and decay-corrected (red) time–activity curves for organs showing measurable signal. Axes vary in scale by row. Shaded area indicates first 5 min of scan, when dynamic images over heart were acquired. %ID = percentage injected dose.
- FIGURE 5.
Summed brain activity images from 20 to 60 min after injection in single participant. Units are SUV based on measured body weight.
- FIGURE 6.
Correlation of 11C-CS1P1 uptake with S1PR1 expression. Regional SUVs were extracted from summed activity image using FreeSurfer and then z-transformed. Matching S1PR1 RNA expression was extracted from Allen Brain Atlas and correlated. Tracer activity and RNA expression correlated significantly, thus providing preliminary evidence that 11C-CS1P1 engages its target.
- FIGURE 7.
Lack of correlation between 11C-CS1P1 uptake and S1PR2–5 expression. Scatterplots in same style as Figure 6 are shown. All correlations are smaller than correlation between 11C-CS1P1 and S1PR1.
Tables
Demographic Participant no. 1 2 3 4 5 6 7 8 9 10 Sex M F F M M M M F F F Age (y) 28 23 40 61 59 34 37 27 27 39 Height (cm) 185.4 177.8 165.1 167.6 185.4 175.3 172.7 165.1 162.6 175.3 Weight (kg) 88.9 105.7 69.4 67.1 104.8 106.1 74.8 117.9 50.3 117.5 Injected dose MBq 266.4 99.9 170.2 247.9 240.5 296.0 225.7 210.9 362.6 144.3 mCi 7.2 2.7 4.6 6.7 6.5 8.0 6.1 5.7 9.8 3.9 Injected mass (μg) 4.70 4.56 3.29 2.88 2.69 3.40 2.94 3.27 2.78 0.98 Site Participant no. Average SD 1 2 3 4 5 6 7 8 9 10 Liver 6.45 5.15 6.85 6.75 6.16 7.41 7.36 4.24 7.76 5.55 6.37 1.11 Brain 0.45 0.39 0.61 0.40 0.38 0.43 0.49 0.29 0.27 0.43 0.41 0.10 Kidney 1.01 0.79 1.22 0.90 0.78 1.15 0.99 0.52 1.09 0.71 0.92 0.22 Small bowel 0.73 0.66 0.83 1.00 0.88 0.77 0.98 0.35 0.74 0.94 0.79 0.19 Lung 0.29 0.24 0.24 0.23 0.20 0.31 0.27 0.21 0.25 0.19 0.24 0.04 Left ventricular wall 0.16 0.85 0.19 0.28 0.27 0.31 0.31 0.12 0.28 0.18 0.30 0.21 Spleen 0.10 0.08 0.12 0.09 0.10 0.10 0.12 0.07 0.07 0.17 0.10 0.03 Gallbladder 0.01 0.02 0.02 0.01 0.01 0.02 0.03 0.01 0.03 0.02 0.02 0.01 Urinary bladder 0.01 0.00 0.02 0.01 0.02 0.06 0.01 0.01 0.01 0.01 0.01 0.02 Remainder 16.82 19.06 16.23 17.68 18.45 16.85 15.51 22.27 16.99 19.65 17.95 1.98 Site Participant no. Mean SD 2 3 8 9 10 Adrenals 5.68 6.56 5.28 6.77 6.07 6.07 0.61 Brain 2.06 2.89 1.75 1.57 2.25 2.10 0.51 Breasts 2.44 2.16 2.62 2.29 2.44 2.39 0.17 Esophagus 3.79 3.47 3.46 3.77 3.52 3.60 0.16 Eyes 2.20 1.98 2.50 1.95 2.28 2.18 0.23 Gallbladder wall 5.88 6.54 5.01 7.28 5.92 6.13 0.84 Left colon 3.47 3.07 3.55 3.21 3.40 3.34 0.20 Small intestine 3.26 3.13 3.46 3.25 3.43 3.31 0.14 Stomach wall 3.50 3.31 3.61 3.48 3.56 3.49 0.11 Right colon 12.40 14.70 8.35 13.50 16.40 13.07 3.03 Rectum 2.82 2.47 3.25 2.57 2.92 2.81 0.31 Heart wall 16.00 5.03 3.93 6.70 4.94 7.32 4.95 Kidneys 14.70 21.90 10.40 20.00 13.70 16.14 4.72 Liver 20.30 26.60 16.90 30.00 21.80 23.12 5.19 Lungs 3.12 3.00 2.73 3.22 2.78 2.97 0.21 Ovaries 2.97 2.64 3.36 2.74 3.08 2.96 0.29 Pancreas 4.75 5.04 4.68 5.39 4.95 4.96 0.28 Salivary glands 2.35 2.09 2.66 2.09 2.42 2.32 0.24 Red marrow 2.62 2.42 2.81 2.54 2.67 2.61 0.15 Osteogenic cells 2.26 2.06 2.48 2.13 2.31 2.25 0.16 Spleen 4.68 6.08 4.08 4.43 7.68 5.39 1.49 Thymus 3.25 2.63 3.13 2.83 2.94 2.96 0.24 Thyroid 2.52 2.18 2.77 2.29 2.52 2.46 0.23 Urinary bladder wall 2.39 2.26 2.86 2.19 2.52 2.44 0.26 Uterus 2.89 2.56 3.30 2.66 3.00 2.88 0.29 Total body 3.30 3.22 3.39 3.37 3.38 3.33 0.07 Effective dose (18) 4.13 4.32 3.69 4.49 4.28 4.18 0.30 Data are single-participant individual organ doses (μSv/MBq).
Site Participant no. Mean SD 1 4 5 6 7 Adrenals 5.71 5.80 5.47 6.28 5.97 5.85 0.30 Brain 1.99 1.80 1.77 1.90 2.09 1.91 0.13 Esophagus 2.69 2.85 2.82 2.90 2.76 2.80 0.08 Eyes 1.62 1.69 1.75 1.62 1.52 1.64 0.09 Gallbladder wall 6.64 7.21 6.50 7.60 7.89 7.17 0.60 Left colon 2.60 2.73 2.75 2.70 2.54 2.66 0.09 Small Intestine 2.70 2.89 2.89 2.80 2.68 2.79 0.10 Stomach wall 2.81 2.95 2.94 2.99 2.84 2.91 0.08 Right colon 13.70 17.80 16.00 14.40 17.40 15.86 1.80 Rectum 2.11 2.22 2.30 2.15 1.98 2.15 0.12 Heart wall 4.07 5.61 5.47 6.14 6.03 5.46 0.83 Kidneys 16.30 14.90 13.10 18.40 16.10 15.76 1.95 Liver 19.90 20.90 19.10 22.80 22.60 21.06 1.63 Lungs 2.55 2.44 2.31 2.78 2.60 2.54 0.18 Pancreas 3.37 3.58 3.52 3.60 3.47 3.51 0.09 Prostate 2.14 2.26 2.33 2.20 2.02 2.19 0.12 Salivary glands 1.84 1.91 1.98 1.84 1.73 1.86 0.09 Red marrow 2.00 2.09 2.12 2.08 1.95 2.05 0.07 Osteogenic cells 1.87 1.95 1.99 1.91 1.79 1.90 0.08 Spleen 4.47 4.17 4.26 4.36 5.00 4.45 0.33 Testes 1.72 1.81 1.89 1.73 1.59 1.75 0.11 Thymus 2.20 2.36 2.39 2.36 2.22 2.31 0.09 Thyroid 1.95 2.03 2.09 1.99 1.84 1.98 0.09 Urinary bladder wall 2.02 2.12 2.36 2.59 1.92 2.20 0.27 Total body 2.39 2.51 2.52 2.50 2.37 2.46 0.07 Effective dose (18) 3.34 3.63 3.44 3.64 3.65 3.54 0.14 Data are single-participant individual organ doses (μSv/MBq).
