Article Figures & Data
Figures
- FIGURE 1.
Cystoscopic injection (needle tip at arrow) into pig bladder of molecular imaging agent IRDye800CW-tilmanocept radiolabeled with 68Ga and 99mTc.
- FIGURE 2.
Fused PET/CT transaxial (A), coronal (B), and sagittal (C) cross-sections of right perivesicular LN (open arrow) adjacent to injection site in bladder wall (solid arrow). Injected dose of 68Ga was 4.4 MBq (120 μCi); at time of PET/CT image this SLN contained 0.085 MBq (2.3 μCi) of radioactivity and had an SUV of 65.2.
- FIGURE 3.
Intraoperative FireFly robotic surgical camera view looking into pelvis with a white light (A) and same location with near-infrared view (B). Fluorescent SLN (open arrow) was visualized within minutes of starting mapping procedure. This SLN contained 88 pmol of fluorescent dye.
Tables
Molecular imaging agent Study no. Tilmanocept (nmol) 68Ga (MBq) 99mTc (MBq) IRDye800CW dye (nmol) Time between administration and SLN mapping (h) No. of SLNs visualized by the endoscopic camera (n) Lymph nodes excised (n) No. of SLNs detected ex vivo by γ-counting* (n) One 1.5 5.5 8.1 2.25 38 2 12 2 Two 1.5 4.4 7.4 2.25 36 1 12 1 Three 1.5 2.9 8.9 2.25 32 2 12 2 ↵* Using 10% rule based on 99mTc radioactivity in cpm.
- TABLE 2
Secondary Objectives: SLN and Injection Site Accumulation During PET/CT and Visualization During Robotic Surgery
Study one Study two Study three PET/CT γ-counter PET/CT γ-counter PET/CT γ-counter Lymph node/injection site SUV Satisfy 10% rule? (yes/no) FireFly in vivo fluorecence? (yes/no) Percentage injected dose (%) Dye content (pmol) SUV Satisfy 10% rule? (yes/no) FireFly in vivo fluorescence? (yes/no) Percentage injected dose (%) Dye content (pmol) SUV Satisfy 10% rule? (yes/no) FireFly in vivo fluorescence? (yes/no) Percentage injected dose (%) Dye content (pmol) L. external iliac No No R. external iliac No No NV Yes 0.38 8.50 Presacral No No No Para aortic No No No L. common iliac No No No R. common iliac 12.4 Yes Yes 2.72 32.3 No 139 Yes Yes 1.33 29.8 L. perivesicular No No No R. perivesicular 59.0 Yes Yes 1.44 61.0 65.2 Yes Yes 3.91 88.0 No L. pelvic No No No R. pelvic No No No L. obturator No No No R. obturator No No No Injection site 2,911 Yes 29.2 658 1,769 Yes 16.5 371 20,322 Yes 64.1 1,441 L. = left; R. = right; NV = not visualized by PET/CT.
If PET/CT or γ-counter did not satisfy the 10% rule, all entries for LN are blank.
