Research ArticleBasic Science Investigations

68Ga-NOTA-UBI-29-41 as a PET Tracer for Detection of Bacterial Infection

Mónica Vilche, Ana Laura Reyes, Elena Vasilskis, Patricia Oliver, Henia Balter and Henry Engler
Journal of Nuclear Medicine April 2016, 57 (4) 622-627; DOI: https://doi.org/10.2967/jnumed.115.161265

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    HPLC profile of 68Ga-NOTA-UBI-29-41.

  • FIGURE 2.
    FIGURE 2.

    Labeling yield for increasing amounts of peptide for starting activity of 42 MBq of 68Ga incubated at 20°C for 10 min (average ± SD; n = 2).

  • FIGURE 3.
    FIGURE 3.

    Percentage of 68Ga-NOTA-UBI-29-41 bound to increasing amount of S. aureus, expressed as percentage of activity bound over total activity (% B/T) vs. colony-forming units (n = 2).

  • FIGURE 4.
    FIGURE 4.

    Biodistribution of 68Ga-NOTA-UBI-29-41 expressed as percentage of activity (%IA) per gram for each organ or tissue in healthy mice (n = 4) at 30, 60, and 120 min after injection (A) and in healthy, inflamed, and infected mice (n = 4) at 60 min after injection (B).

  • FIGURE 5.
    FIGURE 5.

    (A) T/NT for healthy, inflamed, and infected mice at 30, 60, and 120 min after injection, expressed as mean ± SD for at least 4 mice (n = 1 for B). (B) T/NT of infected mice calculated from volumes of interest of PET/CT images at different times after injection.

  • FIGURE 6.
    FIGURE 6.

    PET/CT images of healthy mouse (A), mouse with sterile inflammation (B), and mouse infected with S. aureus (C). Images were acquired 2 h after injection of 68Ga-NOTA-UBI-29-41 (1, coronal; 2, sagittal; and 3, axial). Images correspond to frame 2 of 4. Arrows indicate inflamed or infected muscle.

  • FIGURE 7.
    FIGURE 7.

    S. aureus recovered from infected muscle of mice (n = 13) after injection of 68Ga-NOTA UBI-29-41 correlated with T/NT ratio, expressed as cfu/g. y = 5 × 10−7x + 1.9562 (R2 = 0.0982) for x from 1 × 103 to 1 × 105 cfu/g; and y = 2 × 10−7x + 2.5016 (R2 = 0.7185) for x from 1 × 105 to 1 × 108 cfu/g.

Tables

  • TABLE 1

    Labeling Yield of 68Ga-NOTA-UBI-29-41 at Different Reaction Temperatures, for pH 3.5–4.0, 15–30 nmol of NOTA-UBI-29-41, and 23–38 MBq of 68Ga

    Temperature (°C)% yield*
    2093.7 ± 5.7 (n = 5)
    9095.9 ± 4.4 (n = 5)

    • ↵* Values represent average ± SD of n = 5.

  • TABLE 2

    Percentage of 68Ga-NOTA-UBI-29-41 Bound to Plasma Proteins by Time

    Time (min)% bound to plasma proteins*
    3037.4 ± 0.2
    6044.1 ± 2.6
    9050.6 ± 2.6
    12051.7 ± 2.5

    • ↵* Values are average ± SD of n = 2.

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