Article Figures & Data
Figures
- FIGURE 1.
In vitro characterization of reporter adenovirus. (A) Reporter expression was validated by Western blot in HCT116 cells and by fluorescent cellular immunoassay in PC3-CAR cells 48 h after infection of reporter adenovirus. Supplemental data provide experimental details. (B–D) Functionality of each reporter was verified in HCT116 and PC3-CAR cells by 3H-ganciclovir (HGCV) uptake (B), 125I uptake (C), and YC-I-27 binding (D). Data were normalized to cellular GFP expression and plotted as mean ± SE of mean of 3 replicates. *P < 0.01.
- FIGURE 2.
In vivo comparison of imaging reporters. Mice were contralaterally implanted with 2 Matrigel suspensions of cells expressing either reporter or negative control gene (luciferase) in upper flanks. Luciferase was not used for imaging but as a control gene. After 48 h, each radiotracer was injected and uptake was quantified from regions of interest drawn around the reporter, luciferase control, and muscle. Graphs illustrate time–activity curves for respective reporter-probe systems in the Matrigel suspension model for HSV-sr39tk:18F-FHBG (A), hNIS:125I (C), and PSMA:18F-DCFPyL (E) and reporter-derived signal-to-control and signal-to-muscle ratios for HSV-sr39tk (B), hNIS (D), and PSMA (F) systems, as well as comparison of peak signal-to-noise ratios of each reporter-probe pair (G). Data are mean ± SE of mean of 4 animals. *P < 0.05.
- FIGURE 3.
PET/CT imaging of reporter-probe systems. Representative maximum-intensity projections of reporter-probe systems of data collected from 60 to 90 min after radiotracer injection. (A) Images cropped above liver and below thyroid display the ability of each reporter to sequester its respective probe. (B) Uncropped images demonstrate background associated with each reporter-probe system. All images were decay-corrected and scaled to the same maximum value.
- FIGURE 4.
Monitoring hepatic infection and transgene expression by imaging PSMA in a murine model. Athymic nude mice were intravenously administered adenovirus expressing PSMA or negative control gene (luciferase [Luc]). Seventy-two hours after injection of adenovirus, animals were imaged and specific uptake was observed in liver of animals by optical (A) and SPECT (B) imaging. SPECT images were obtained 15 min after injection of radiotracer, with similar levels of radioactivity in liver at 4 h after injection (Supplemental Fig. 4).
Tables
- TABLE 1
Description of Reporter Adenoviruses and Respective Imaging Modalities Used for Detection of Reporter Genes
Virus Reporter gene Imaging modality Ad-Track-PSMA Prostate-specific membrane antigen Optical, PET, SPECT Ad-Track-hNIS Human sodium iodide symporter PET, SPECT Ad-HSV-sr39tk Herpes simplex virus mutant thymidine kinase PET
Supplemental Data
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