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Research ArticleClinical Investigation

Correlation of In Vivo and In Vitro Measures of Carbonic Anhydrase IX Antigen Expression in Renal Masses Using Antibody 124I-cG250

Daniel A. Pryma, Joseph A. O'Donoghue, John L. Humm, Achim A. Jungbluth, Lloyd J. Old, Steven M. Larson and Chaitanya R. Divgi
Journal of Nuclear Medicine April 2011, 52 (4) 535-540; DOI: https://doi.org/10.2967/jnumed.110.083295
Daniel A. Pryma
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Joseph A. O'Donoghue
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John L. Humm
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Achim A. Jungbluth
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Lloyd J. Old
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Steven M. Larson
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Chaitanya R. Divgi
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  • FIGURE 1.
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    FIGURE 1.

    (A and B) Axial, sagittal, and coronal CT (top), PET (middle), and fused PET/CT (bottom) images of patient with clear cell renal cancer with relatively homogeneous distribution of antigen (arrows) (A) and patient with large, centrally necrotic clear cell renal cancer with marked heterogeneity of antigen distribution within mass (arrows) (B). (C) Axial CT (top), PET (middle), and fused PET/CT (bottom) images of patient with advanced clear cell renal cancer. Antigen distribution within primary tumor is heterogeneous (arrows), whereas distribution within metastatic nodes is relatively homogeneous (arrowheads).

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    FIGURE 2.

    Autoradiographic (A) and histologic (B) sections from patient with clear cell renal cancer. Marked heterogeneity of antibody binding seen on autoradiography correlates with areas of densest tumor cells on histology.

Tables

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    TABLE 1

    Measured %ID/g for Each Subject Using Digital Autoradiography, Well Counting, and PET/CT

    SubjectAutoradiographyWell countingPET/CT
    10.06810.03340.0453
    20.00230.00150.0039
    30.00180.00060.0066
    4*———
    50.01300.00940.0179
    60.04520.03800.0255
    70.14770.06570.0481
    80.12690.06020.0391
    90.01720.04130.0444
    100.00930.00530.0100
    110.13700.06620.0503
    120.09540.03690.0268
    130.03950.01240.0378
    140.00320.00030.0173
    150.00960.00390.0382
    160.00350.00220.0061
    170.01450.00430.0288
    180.00620.00370.0177
    190.00500.00210.0125
    200.00130.00060.0028
    210.00150.00120.0097
    220.00460.00200.0074
    230.03390.02580.0414
    240.00250.00150.0035
    250.00180.00080.0035
    260.00910.01950.0219
    • ↵* Subject 4 received immunologically inactive antibody and was excluded from analysis.

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Journal of Nuclear Medicine: 52 (4)
Journal of Nuclear Medicine
Vol. 52, Issue 4
April 1, 2011
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Correlation of In Vivo and In Vitro Measures of Carbonic Anhydrase IX Antigen Expression in Renal Masses Using Antibody 124I-cG250
Daniel A. Pryma, Joseph A. O'Donoghue, John L. Humm, Achim A. Jungbluth, Lloyd J. Old, Steven M. Larson, Chaitanya R. Divgi
Journal of Nuclear Medicine Apr 2011, 52 (4) 535-540; DOI: 10.2967/jnumed.110.083295

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Correlation of In Vivo and In Vitro Measures of Carbonic Anhydrase IX Antigen Expression in Renal Masses Using Antibody 124I-cG250
Daniel A. Pryma, Joseph A. O'Donoghue, John L. Humm, Achim A. Jungbluth, Lloyd J. Old, Steven M. Larson, Chaitanya R. Divgi
Journal of Nuclear Medicine Apr 2011, 52 (4) 535-540; DOI: 10.2967/jnumed.110.083295
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Cited By...

  • Evaluation of Nonpeptidic Ligand Conjugates for the Treatment of Hypoxic and Carbonic Anhydrase IX-Expressing Cancers
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  • Radretumab Radioimmunotherapy in Patients with Brain Metastasis: A 124I-L19SIP Dosimetric PET Study
  • Advances in Immuno-Positron Emission Tomography: Antibodies for Molecular Imaging in Oncology
  • Bone Marrow Dosimetry Using 124I-PET
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