Research ArticleBasic Science Investigations

Multimodal Imaging with 18F-FDG PET and Cerenkov Luminescence Imaging After MLN4924 Treatment in a Human Lymphoma Xenograft Model

Robbie Robertson, Melissa Saylor Germanos, Mark G. Manfredi, Peter G. Smith and Matthew D. Silva
Journal of Nuclear Medicine November 2011, 52 (11) 1764-1769; DOI: https://doi.org/10.2967/jnumed.111.091710

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Figures

  • FIGURE 1.
    FIGURE 1.

    PET images from representative pairs of animals. Reduction in signal intensity can be observed after treatment, indicating reduction in uptake of 18F-FDG in tumor. Arrows refer to tumor location.

  • FIGURE 2.
    FIGURE 2.

    MLN4924 treatment results in reduction in tumor volume from baseline of 56% on day 3 and 84% on day 13 in OCI-Ly10 xenograft model. Treatment was halted on day 15 of study, and volume reduction is durable when compared with both baseline (−64%) and vehicle group (−87%). *P < 0.05. **P < 0.01. ***P < 0.001.

  • FIGURE 3.
    FIGURE 3.

    SUVave (A) and decay-corrected %ID/cm3 (B). On day 13, all PET parameters are significantly reduced, as compared with vehicle treatment, by 21.3% and 25.6% for SUVave and %ID/cm3, respectively (P < 0.0001). ***P < 0.001.

  • FIGURE 4.
    FIGURE 4.

    Cerenkov luminescence images from representative pairs of animals. As observed on PET data, there is visual reduction in image signal intensity in MLN4924 treatment group that is consistent with reduction in 18F-FDG uptake (Fig. 1).

  • FIGURE 5.
    FIGURE 5.

    Cerenkov luminescence image analysis: radiance (A) and radiance/ID (B). On day 13, CLI parameters for MLN4924-treated group were all significantly reduced from baseline (30.9% and 30.4%) and from vehicle-treated values (37.5% and 37.2%) for radiance and radiance/ID, respectively (all P < 0.0001). **P < 0.01. ***P < 0.001.

  • FIGURE 6.
    FIGURE 6.

    There was strong correlation between PET %ID/cm3 and CLI radiance/ID (r2 = 0.82). Correlations between PET %ID/cm3 and CLI radiance (r2 = 0.83) and other parameters are shown in Supplemental Figure 5.

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