Article Figures & Data
Figures
- FIGURE 1.
Macroscopic (A) and microscopic (B) observations of indomethacin-induced small-intestine ulceration during healing in rats. (A) Continuous circular lesions were seen on side of mesenteric attachment on day 1 or 4. (B) Histologically, denuded epithelium and severe edema in submucosa (day 1), granulation tissue on ulcer bed (day 4), and neoepithelial cells (day 7) were observed (hematoxylin and eosin staining; scale bar, 200 μm). (C) Change in myeloperoxidase activity as marker of neutrophil infiltration during indomethacin-induced intestinal ulceration and healing. Myeloperoxidase activity was evaluated in animals after administration of indomethacin (days 1, 2, 4, and 7) and in vehicle-injected animals (control). Data are shown as mean ± SD; n = 4–5 animals. *P < 0.05.
- FIGURE 2.
γ-counting study of 18F-FDG uptake in ulcerated intestine, showing 18F-FDG accumulation in small intestine at time points after indomethacin administration (days 1, 2, 4, and 7) and after vehicle injection (control). Data are shown as mean ± SD; n = 6–8 animals for each time point.
- FIGURE 3.
18F-FDG PET study of indomethacin-induced intestinal ulceration. (A) Abdominal PET images (coronal images) in same rat at different times after indomethacin administration. Arrowheads indicate characteristic accumulation of 18F-FDG. (B) Quantification of 18F-FDG uptake in PET study. Mean SUVs are shown as mean ± SD; n = 4 animals. *P < 0.05.
- FIGURE 4.
18F-FDG macroautoradiography of intestinal ulceration. Rats were intravenously injected with 18F-FDG and euthanized 45 min later. Discontinuous dotlike 18F-FDG accumulation was clearly identified mainly in ileum on day 1. 18F-FDG imaging profile was similar to that of control on day 7. Images at far right show plain photograph (upper) and 18F-FDG image (lower) of intestines opened along longitudinal axis for control and at day 1. D = duodenum; C = cecum.
- FIGURE 5.
18F-FDG microautoradiography of indomethacin-induced intestinal lesions combined with hematoxylin and eosin staining. (A–C) At 1 d after administration of indomethacin, silver grains of high density were observed in ulcerated area, especially at ulcer margin (B) and submucosal and smooth muscle layer (C). B and C are magnified views of areas indicated by arrows B and C in A. (D and E) On day 4, grains were accumulated on cells forming granulation tissue. (E) Magnified view of the region indicated by arrow E in D. Scale bars: 100 μm (A and D) and 20 μm (B, C, and E).
- FIGURE 6.
Microautoradiography combined with immunohistochemistry. In submucosal area at day 1 after indomethacin administration, 18F-FDG uptake was observed mainly in peroxidase-positive cells (A and B). On granulation tissue at day 4, myofibroblasts (C; α-SMA–positive), macrophages (D; ED2-positive), and endothelial cells (E; CD31-positive) were visualized. In normal tissue, silver grains of high density reflecting 18F-FDG radioactivity were observed in Ki67-positive crypt cells (F). Insets show magnified view of region indicated by arrowheads in each panel. Scale bars: 100 μm (A), 10 μm (B), and 5 μm (C–F).
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