Article Figures & Data
Figures
- FIGURE 1.
Localization of TN (rat 10): Coronal brain slice in the anatomic map (A) was identified using the histologic slice (C). The corresponding autoradiogram of d-cis-18F-FPro (B) was adapted to the circumference of this map. Reprojection of focal tracer uptake in the midbrain to the anatomic map identifies the thalamic nucleus. The ventroposteromedial thalamic nucleus (VPM) is indicated by an ellipse. The corresponding autoradiogram using 3H-PK11195 (D) shows only minor tracer uptake in the VPM. (Image A modified and reprinted with permission of (15).)
- FIGURE 2.
d-cis-18F-FPro uptake in TN (ratio of tracer uptake in thalamic tissue divided by normal brain tissue) vs. time after cortical infarction. There is a significant correlation.
- FIGURE 3.
(A) Dorsal view of rat brain 7 d after PT (rat 6). Cortical infarction involves parts of motor and somatosensory cortex. (B) Sagittal slice from anatomic rat brain atlas demonstrates position of infarction (stippled area) and position of coronal slices: 1, level of basal ganglia (C); 2, level of hippocampus/thalamus (D). Autoradiogram with d-cis-18F-FPro (C, left image) exhibits tracer uptake in area of cortical infarction and in caudate nucleus (arrow). Furthermore, there is focal d-cis-18F-FPro uptake in ventral posteromedial thalamic nucleus and posterior thalamic nuclear group (D, left image, arrows). Corresponding autoradiograms using 3H-DG (C and D, middle images) and histologic staining using toluidine blue (C and D, right images) show no abnormalities in caudate nucleus and in thalamus. (Image B modified and reprinted with permission of (15).)
- FIGURE 4.
(A) Dorsal view of rat brain 7 d after PT (rat 5) with infarction in visual cortex. (B) Sagittal slice from anatomic rat brain atlas demonstrates position of infarction (stippled area) and position of coronal slices: 1, level of the basal ganglia (C); 2, level of the hippocampus/thalamus (D). Autoradiograms with d-cis-18F-FPro (C and D, left images) exhibit tracer uptake in area of cortical infarction and in lateral posterior thalamic nucleus (LP) and dorsolateral geniculate nucleus (DLG) (D, arrow). Corresponding autoradiograms using 3H-DG (C and D, middle images) and histologic staining using toluidine blue (C and D, right images) show no abnormalities in thalamus. (Image B modified and reprinted with permission of (15).)
- FIGURE 5.
(A–C) Coronal slices of left hemisphere at level of thalamus 28 d after PT (rat 11). (A) Autoradiogram using d-cis-18F-FPro shows high uptake in posterior thalamic nuclear group (arrow). (B) Immunofluorescent staining using CD11b shows activated microglia (arrow). (C) Immunofluorescent staining using GFAP shows reactive astrogliosis in same area (arrow) as in B. (D) Thalamic nucleus at higher magnification: blue = cell nuclei; red = astrocytes; green = activated microglia.
- FIGURE 6.
Cortical infarction 7 d after PT. (A and B) Autoradiographic distribution of d-cis-18F-FPro (A) and 3H-DG (B). (C) Double immunofluorescence labeling using CD11b shows activated microglia (green) and GFAP shows reactive astrogliosis (red). (D) Immunofluorescence labeling using CD68 shows macrophages. d-cis-18F-FPro and 3H-DG accumulation is similar to high density of activated microglia and macrophages but different from that of reactive astrocytosis.
Tables
d-cis-FPro uptake 3H-DG 3H-PK11195 3H-MET Rat no. Time after PT (d) Cortical area Thalamic nucleus, striatum PT/brain TN/brain PT/brain TN/brain PT/brain TN/brain PT/brain TN/brain 1 7 Mot, Sens CPu, Po, VPM, VPL 12.6 4.0 2 5 Mot, Sens CPu, Po, VL 11.7 6.3 4.2 1.4 3 8 Vis DLG, LP 16.6 8.0 2.4 1.0 4 7 Sens, Vis Po, VPM, DLG 12.4 6.0 5.5 1.0 5 7 Vis DLG, LP 17.6 3.4 2.2 1.1 6 7 Mot, Sens CPu, Po, VPM, VPL, VL 9.4 4,0 3.0 1.2 7 7 Mot CPu, VL 16.8 4.8 3.0 1.0 8 7 Sens Po, VPM, VPL 10.7 9.0 2.7 0.9 9 7 Sens Po, VPM, VPL 6.6 5.6 2.3 0.8 10 14 Sens Po, VPM, VPL 31.6 11.8 5.7 1.4 11 28 Sens Po, VPM 4.1 9.6 12 28 Sens Po, VPM 24.8 13.4 13 28 Sens Po, VPM 7.0 9.3 8.0 1.0 14 5 Mot, Sens CPu, Po, VPM 11.8 3.5 2.7 1.1 15 3 Mot, Sens CPu, Po, VPM 13.4 3.4 1.0 0.8 16 3 Mot, Sens CPu, Po, VPM 20.8 2.4 0.6 0.9 17 2 Sens — 3.3 — 0.9 — 18 2 Sens — 3.8 — 0.7 — 19 1 Sens — 3.4 — 1.0 — 20 1 Sens — 3.8 — 0.6 — Mean 12.1 6.7 2.5 1.0 6.8 1.2 1.4 1.0 SD 8.1 3.5 1.5 0.2 1.7 0.3 1.1 0.2 PT = cortical infarction induced by photothrombosis; ; 3H-DG = 3H-deoxyglucose; 3H-Pk11195 = ligand for peripheral benzodiazepine receptors; 3H-MET = 3H-methyl-l-methionine; PT/brain = ratio of maximal tracer uptake PT divided by mean uptake in normal brain tissue; TN/brain = ratio of maximal tracer uptake in thalamic nucleus divided by mean uptake in normal brain; Mot = motor cortex; Sens = somatosensory cortex; CPu = caudate putamen; Po = posterior thalamic nuclear group; VPM = ventral posteromedial thalamic nucleus; VPL = ventroposterolateral thalamic nucleus; VL = ventrolateral thalamic nucleus; Vis = visual cortex; DLG = dorsal lateral geniculate nucleus; LP = lateral posterior thalamic nucleus.
