OtherBASIC SCIENCE INVESTIGATIONS

Effect of Postconditioning on Myocardial 99mTc-Annexin-V Uptake: Comparison with Ischemic Preconditioning and Caspase Inhibitor Treatment

Junichi Taki, Takahiro Higuchi, Atsuhiro Kawashima, Makoto Fukuoka, Daiki Kayano, Jonathan F. Tait, Ichiro Matsunari, Kenichi Nakajima, Seigo Kinuya and H. William Strauss
Journal of Nuclear Medicine August 2007, 48 (8) 1301-1307; DOI: https://doi.org/10.2967/jnumed.106.037408

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Experimental protocol. Control rats (A), rats with ischemic postconditioning (B), rats with ischemic preconditioning (C), and rats with caspase inhibitor treatment (D).

  • FIGURE 2. 
    FIGURE 2. 

    Autoradiography of 99mTc-annexin-V and 201Tl. After 20 min of ischemia, 99mTc-annexin-V was injected at 30 or 90 min after reperfusion. Single mid-ventricular slices are shown from representative animals from each group. 201Tl images demonstrate area at risk. Intense 99mTc-annexin-V uptake is observed in area at risk in control rat; however, uptake was markedly decreased by postconditioning and preconditioning and by caspase inhibitor treatment.

  • FIGURE 3. 
    FIGURE 3. 

    99mTc-Annexin-V uptake ratios in control rats (cont) and in rats with postconditioning (poscon), preconditioning (precon), and caspase inhibitor. 99mTc-Annexin-V uptake ratio was calculated by dividing 99mTc-annexin-V count density in area at risk by that in nonischemic area. Reperfusion time indicates time of 99mTc-annexin-V injection after reperfusion. Compared with control rats, all 3 interventional groups demonstrated significant reduction of 99mTc-annexin-V uptake.

  • FIGURE 4. 
    FIGURE 4. 

    Percentage of TUNEL-positive cardiomyocytes in control rats (cont) and in rats with postconditioning (poscon), preconditioning (precon), and caspase inhibitor. Myocardial percentage of TUNEL-positive staining is markedly depressed by postconditioning, preconditioning, and caspase inhibitor, compared with control rats (P < 0.0001).

  • FIGURE 5. 
    FIGURE 5. 

    TUNEL staining. (A) Control rat. Several cardiomyocytes have become TUNEL-positive (brown staining of nucleus) after ischemia and 90 min of reperfusion. (B) Postconditioning rat. Small number of cardiomyocytes showed TUNEL-positive staining after ischemia and 90 min of reperfusion. (C) Preconditioning rat. Minimal numbers of cardiomyocytes demonstrated TUNEL-positive staining after ischemia and 90 min of reperfusion. (D) Rat with caspase inhibitor. Minimal numbers of cardiomyocytes demonstrated TUNEL-positive staining after ischemia and 90 min of reperfusion.

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