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Preclinical Safety Evaluation of 18F-FHBG: A PET Reporter Probe for Imaging Herpes Simplex Virus Type 1 Thymidine Kinase (HSV1-tk) or Mutant HSV1-sr39tk's Expression

Shahriar S. Yaghoubi, Marcelo A. Couto, Chang-Cheng Chen, Lathasree Polavaram, Guanggen Cui, Luyi Sen and Sanjiv S. Gambhir
Journal of Nuclear Medicine April 2006, 47 (4) 706-715;

Article Figures & Data

Figures

  • FIGURE 1. 
    FIGURE 1. 

    Average rat BP ± SEM before and after test article injection. Group 5A was control males, group 6A was FHBG males, group 5B was control females, and group 6B was FHBG males. n = 5 for all groups. (A) Systolic BP. Significant differences were observed between averages of FHBG and control males on day −1 (P < 0.001), day 0 before injection (P < 0.005), and day 0 after injection (P < 0.02) but not on other days. Means of FHBG and control females are significantly different on day 14 (P < 0.01) but not on any other days. (B) Mean BP. Significant differences were observed between averages of FHBG and control males on day −1 (P < 0.001), day 0 before injection (P < 0.05), and day 1 (P < 0.05) but not on other days. Averages of FHBG and control females are significantly different only on day 1 (P < 0.002). ND = not determined; BI = before injection; AI = after injection.

  • FIGURE 2. 
    FIGURE 2. 

    (A) Average rabbit systolic BP ± SEM on days −1, 1, and 14. (B) Average rabbit diastolic BP ± SEM on days −1, 1, and 14. No significant differences were observed between systolic or diastolic BP of FHBG and control females or males. n = 4 for FHBG males and n = 3 for all other groups.

  • FIGURE 3. 
    FIGURE 3. 

    Electrocardiograph PR interval (A), QRS complex (B), and QT interval (C) measured in seconds. Data are averages ± SEM. QT intervals of FHBG and control male rabbits are significantly different on days −1 (P < 0.05) and 14 (P < 0.02). No other significant differences were observed. n = 4 for FHBG males and n = 3 for all other groups.

  • FIGURE 4. 
    FIGURE 4. 

    Food (A) and water (B) consumption of rats before and after test article injections. Data are averages (n = 5).

  • FIGURE 5. 
    FIGURE 5. 

    Food (A) and water (B) consumption of rabbits before and after test article injections. Data are averages (n = 4 for FHBG males and n = 3 for all other groups).

Tables

  • TABLE 1

    Procedure Schedule for Rats

    123456
    Group Day−101714−101714−101714−101714−101714−101714
    FHBG (μg/kg)014014014
    Blood chemistry and hematologyXXXXXXX
    UrinalysisXXXXXXX
    Respiratory rateXXXXXXXXXX
    Pulse oximetryXXXXXXXXXX
    ECGXXXXXXXXXX
    Blood pressure (tail cuff)XXXXXXXXXX
    Blood pressure (direct femoral artery)XX
    Weight measurementXXXXXXXXXXXXXXXXXX
    NecropsyXXXXXX
    HistopathologyXX

    • ECG=electrocardiogram.

  • TABLE 2

    Average Rabbit Body Temperatures

    RabbitsBefore injection<1 h after injection>1 h after injectionDay 7
    Control male38.62 ± 0.1139.08 ± 0.1338.93 ± 0.1338.87 ± 0.09
    FHBG male38.53 ± 0.2538.80 ± 0.1938.95 ± 0.0638.73 ± 0.09
    Control female39.3 ± 0.1039.47 ± 0.0939.00 ± 0.3239.03 ± 0.09
    FHBG female39.47 ± 0.1239.43 ± 0.1239.27 ± 0.0939.10 ± 0.15

    • Temperatures (°C) are expressed as averages ± SEM.

Additional Files

  • Supplemental Data

    Supplemental Data

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