Article Figures & Data
Figures
- FIGURE 1.
Pharmacokinetics of 18F-FBPA for F98 glioma-bearing rats determined by PET. (A) Time–activity curves of 18F-FBPA in tumors and ipsilateral brains of F98 glioma-bearing rats derived from dynamic PET images after intracarotid (i.c.) injection with and without BBB-D and after intravenous (i.v.) injection. (B) Tumor-to-ipsilateral brain ratio of 18F-FBPA.
- FIGURE 2.
Time–activity curves of 18F-FBPA in blood of F98 glioma-bearing Fischer 344 rats after intracarotid (i.c.) injection with and without BBB-D. Data points were fitted with open 2-compartment model (Cp = Ae−αt + Be−βt). r2 = 0.97–0.99.
- FIGURE 3.
Biodistribution of 10B-BPA for F98 glioma-bearing rats. (A) Boron concentration in tumor, ipsilateral brain, contralateral brain, and blood of F98 glioma-bearing Fischer 344 rats after intracarotid injection of BPA (300 mg/kg) with BBB-D. (B) Tumor-to-normal tissue ratio of boron concentration. Rats were under ketamine mixture anesthesia.
- FIGURE 4.
Boron concentration in tumor, ipsilateral brain (IB), contralateral brain (CB), and blood at 2.5 h after administration of 500 mg/kg of BW of BPA under isoflurane/ketamine mixture anesthesia.
- FIGURE 5.
Correlation among tumor region of interest (ROI), k12/k21, and tumor-to-ipsilateral brain ratio. (A) Correlation between radioactivity of tumor ROI and k12/k21. (B) Correlation between tumor-to-ipsilateral brain ratio and k12/k21. Each point represents one rat. Radioactivity of tumor ROI and tumor-to-ipsilateral brain ratio were derived from PET images at 2.5 h after intracarotid injection of 18F-FBPA with and without BBB-D. k12/k21 ratio was obtained from pharmacokinetic analysis of blood of the same rat.
Tables
- TABLE 1
Estimated Pharmacokinetic Parameters Derived from Time–Activity Curves of Blood of F98 Glioma-Bearing Fischer 344 Rats After Intracarotid (i.c.) Injection of 18F-FBPA With and Without BBB-D
Parameter i.c. i.c. + BBB-D kel (min−1) 0.0071 ± 0.0014 0.0206 ± 0.0018 k12 (min−1) 0.0160 ± 0.0017 0.0260 ± 0.0016 k21 (min−1) 0.0061 ± 0.0004 0.0039 ± 0.0003 V1 (mL) 3.6 ± 0.1 3.1 ± 0.1 k12/k21 2.6 ± 0.2 6.7 ± 0.5 Each point represents mean ± SD of 6 rats.
All P values for comparison of kel, k12, k21, V1, and k12/k21 were <0.01.
- TABLE 2
Biodistribution of 18F-FBPA in Tumor (T), Ipsilateral Brain (IB), Contralateral Brain (CB), and Blood (Bd) of F98 Glioma-Bearing Fischer 344 Rats at 0.5, 2.5, and 4.0 Hours after Intracarotid Administration of 18F-FBPA (29.6 MBq/kg of BW) with BBB-D
Time (h) Accumulation (%ID/g) Ratio T IB CB Bd T/IB T/CB T/Bd 0.5 3.32 ± 0.92 1.15 ± 0.44 0.87 ± 0.20 1.72 ± 0.15 2.9 3.8 1.9 2.5 2.63 ± 0.72 0.55 ± 0.21 0.46 ± 0.12 0.39 ± 0.08 4.8 5.7 6.7 4.0 1.48 ± 0.25 0.42 ± 0.08 0.24 ± 0.04 0.18 ± 0.06 3.5 5.9 7.9 Each point represents mean ± SD of 4 or 5 rats. Rats were under isoflurane anesthesia.
- TABLE 3
Boron Concentration in Tumor (Tumor [T] Implanted in Left Brain Region), Ipsilateral Brain (IB), Contralateral Brain (CB), and Blood (Bd) of F98 Glioma-Bearing Fischer 344 Rats at 2.5 Hours After Administration of BPA (500 mg/kg) by Various Routes
Route Boron concentration (μg/g) Ratio T IB CB Bd T/IB T/CB T/Bd i.c. + BBB-D 76.6 ± 12.1 12.1 ± 3.5 10.1 ± 1.5 9.2 ± 2.4 6.3 7.6 8.3 i.c. 43.1 ± 5.6 10.5 ± 0.9 9.3 ± 0.2 9.5 ± 1.4 4.1 4.6 4.5 i.v. 19.5 ± 4.5 7.8 ± 1.4 8.4 ± 1.6 9.0 ± 1.2 2.5 2.3 2.2 i.c. = intracarotid; i.v. = intravenous.
Each point represents mean ± SD of 5 or 6 rats. Rats were under ketamine mixture anesthesia.
- TABLE 4
Biodistribution of 18F-FBPA and BPA in Tumor (T), Ipsilateral Brain (IB), Contralateral Brain (CB), and Blood (Bd) of F98 Glioma-Bearing Fischer 344 Rats at 2.5 Hours After Intracarotid Injection of a Mixture of 18F-FBPA (29.6 MBq/kg) and BPA (300 mg/kg) with BBB-D
Element Accumulation Ratio T IB CB Bd T/IB T/CB T/Bd 18F (%ID/g) 4.63 ± 0.72 0.61 ± 0.08 0.56 ± 0.12 0.52 ± 0.08 7.6 8.3 8.9 10B (μg/g) 45.5 ± 3.4 6.3 ± 0.23 5.6 ± 0.12 5.5 ± 0.33 7.2 8.1 8.3 Each point represents mean ± SD of 3 rats. Rats were under ketamine mixture anesthesia.
