Article Figures & Data
Figures
- FIGURE 1.
Cell cycle changes 48 h after EGT ATS on CT26 cells. Representative flow cytometry graphs of cells 48 h after in vitro EGT with control plasmid (A), and cells 48 h after in vitro EGT with ATS (B). There was an increased S-phase blockage of CT26 cells 48 h after EGT ATS.
- FIGURE 2.
Thymidylate synthase assay 48 h after in vitro EGT ATS on CT26 cells. TS catalytic activity was determined as described and was measured in femtomoles per milligram per hour. The TS catalytic activity of CT26 cells was decreased 40% 48 h after in vitro EGT with ATS compared with cells treated with the control plasmid. Data represent mean data of 3 experiments. All data shown are mean ± SD.
- FIGURE 3.
Effect of in vitro EGT ATS on incorporation of 131I-IdUrd DNA in CT26 cells. CT26 cells were treated with either control plasmid or ATS by in vitro electroporation 45 h before adding 0.37 MBq/mL 131I-IdUrd for another 3 h. Counted to equal cell number, DNA was extracted by PCA precipitation, and pellets were counted for radioactivity. Bars represent mean ± SD of 3 independent experiments in triplicate. Results are represented as counts per minute per 105 cells.
- FIGURE 4.
In vivo EGT ATS on the enhancement of antitumoral activity of 131I-IdUrd or 5-FU against established CT26 tumor. Mice bearing subcutaneous tumors 4 mm in diameter were randomized into 7 groups and treated in situ with EGT of control plasmid or ATS on days 1, 2, and 3, with or without 50 mg/kg 5-FU injected intraperitoneally on day 3, with implantation of osmotic minipumps containing 11.1 MBq 131I-IdUrd or 200 μL PBS from day 3 to day 10. Each symbol represents mean ± SD tumor volume from 5 mice per group. *P < 0.05.
- FIGURE 5.
Biodistribution of 131I-IdUrd in tumor 14 d after implantation of miniosmotic pumps. CT26 tumors were in vivo EGT with control plasmid or ATS for 3 d before implantation of 131I-IdUrd miniosmotic pump with or without 50 mg/kg 5-FU injected intraperitoneally on day 3. Black columns represent total radioactivity count for indicated treatment groups. White columns represent percentage injected dose per gram of tumor.
