OtherBasic Science Investigations

Augmented 18F-FDG Uptake in Activated Monocytes Occurs During the Priming Process and Involves Tyrosine Kinases and Protein Kinase C

Jin-Young Paik, Kyung-Han Lee, Yearn Seong Choe, Yong Choi and Byung-Tae Kim
Journal of Nuclear Medicine January 2004, 45 (1) 124-128;

Article Figures & Data

Figures

  • FIGURE 1.
    FIGURE 1.

    Oxygen-intermediate generation. (A) Serial 30-s luminescence levels from monocytes that were unprimed (▪), IFN-γ primed (▵), and PMA stimulated after priming (•). (B) Cumulative luminescence levels from monocytes. Results are mean ± SD of triplicate samples obtained from a single experiment representative of 3 separate experiments.

  • FIGURE 2.
    FIGURE 2.

    Relative 18F-FDG uptake in monocytes that were unprimed, IFN-γ primed, and PMA stimulated after priming. Results are expressed as percentage uptake relative to the mean of unprimed control cells. Data are mean ± SD of triplicate samples obtained from a single experiment representative of 3 separate experiments.

  • FIGURE 3.
    FIGURE 3.

    Effect of staurosporine and genistein on 18F-FDG uptake in unprimed control monocytes and IFN-γ primed monocytes. Results are percentage uptake relative to the mean of unprimed cells. Data are mean ± SD of triplicate samples from single experiment representative of 2 separate experiments. P value is for comparison with monocytes primed in absence of inhibitors.

  • FIGURE 4.
    FIGURE 4.

    Effect of wortmannin and cycloheximide on 18F-FDG uptake in monocytes. Results are percentage uptake relative to the mean of unprimed cells. Data are mean ± SD of triplicate samples from a single experiment representative of 2 separate experiments. P value is for comparison with unprimed control monocytes.

  • FIGURE 5.
    FIGURE 5.

    Schematic representation of experimental design and results. Purified human monocytes that were activated with PMA after priming with IFN-γ showed enhanced 18F-FDG uptake and respiratory-burst activation. Priming alone did not stimulate respiratory-burst activity but was sufficient to enhance 18F-FDG uptake. This effect was completely abolished by staurosporine and genistein, inhibitors of protein kinase C (PKC) and tyrosine kinases (TyrK), respectively, whereas wortmannin, a PI3 kinase inhibitor, and cycloheximide, a protein synthesis (Psyn) inhibitor, had little effect.

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