Article Figures & Data
Figures
- FIGURE 1.
Stained tissues (hematoxylin-eosin) of turpentine-induced abscesses in normal, immunocompetent rabbit (A) and neutropenic rabbit (B). Note massive infiltration of polymorphonuclear cells (neutrophils) in A and mononuclear cells in B. (Hematoxylin-eosin, ×400 and ×1,000 [inset A, B])
- FIGURE 2.
Images of rabbits with turpentine-induced abscesses in left thigh muscle at 5 min (0 h) and 1, 2, and 4 h after injection of 99mTc-IL-8. Neutrophil counts varied from very low levels (Neutropenic rabbit) via low levels (Semineutropenic rabbit) to normal levels (Normal rabbit).
- FIGURE 3.
Blood clearance of 99mTc-IL-8 in normal, immunocompetent rabbits and in neutropenic rabbits with turpentine-induced inflammation. Data are expressed as %ID in blood pool.
- FIGURE 4.
Kinetics of in vivo distribution of 99mTc-IL-8 in rabbits with E. coli-induced infection as determined by quantitative analysis of images. Uptake is quantified as percentage of whole-body activity. Error bars indicate ±SEM.
- FIGURE 5.
Detailed ex vivo analysis of uptake of 99mTc-IL-8 in E. coli-infected hindleg of New Zealand White rabbit at 6 h after injection. Uptake is given as %ID and as %ID/g for central parts of the affected muscle (compare with in vivo data, Table 3).
- FIGURE 6.
Typical example of ROIs in image of rabbit with E. coli-induced intramuscular infection injected with 99mTc-IL-8.
Tables
- TABLE 1
Biodistribution of 99mTc-IL-8 in Neutropenic and Normal Rabbits with Turpentine-Induced Abscesses
Biodistribution Neutropenic rabbits (n = 3) Normal rabbits (n = 4) Blood 0.073 ± 0.004 0.069 ± 0.004 Muscle 0.005 ± 0.001 0.004 ± 0.001 Abscess 0.038 ± 0.014 0.41 ± 0.05 Lung 0.10 ± 0.02 0.34 ± 0.04 Spleen 0.38 ± 0.04 0.90 ± 0.08 Kidney 2.32 ± 0.30 2.13 ± 0.22 Liver 0.110 ± 0.024 0.080 ± 0.006 Intestine 0.044 ± 0.008 0.025 ± 0.002 Ratio Abscess/blood 0.5 ± 0.2 5.9 ± 0.7 Abscess/muscle 10 ± 5 110 ± 10 Target/background 1.2 ± 0.1 15 ± 1 -
Uptake is given as %ID/g. Values are expressed as mean ± 1 SEM.
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- TABLE 2
Distribution of 99mTc-IL-8 over Red Blood Cells, White Blood Cells, and Plasma in Normal Rabbits with Turpentine-Induced Abscesses
Time after injection Plasma (%) RBCs (%) WBCs (%) 5 min 68 ± 2 31 ± 2 0.3 ± 0.02 2 h 68 ± 4 30 ± 4 2.3 ± 0.2 4 h 76 ± 4 23 ± 4 0.8 ± 0.2 -
RBC = red blood cell; WBC = white blood cell.
From blood samples at 3 different time points. Values are expressed as mean ± 1 SEM.
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- TABLE 3
Detailed Analysis of Uptake of 99mTc-IL-8 in E. coli-Infected Hindleg of New Zealand White Rabbit
Dissected parts Ex vivo data (%ID) ROI In vivo data (% whole body) Abscess core 3.8 Abscess core 4.5 Muscle part 11 Abscess peripheral 12.2 Upper hindleg 17.6 Abscess total 18.5 Kidney, left 14.4 Kidney, left 13.2 Kidney, right 15.4 Kidney, right 15.1 Liver 8.6 Liver 9.7 Spleen 0.75 Spleen 0.60 Lungs 4.4 Lungs 3.9 -
Comparison of radioactivity uptake in whole unfragmented organs and abscess parts (ex vivo analysis; Fig. 5) with uptake as determined by quantitative analysis of images at 6 h after injection. Ex vivo data are expressed as %ID; in vivo data are expressed as percentage of WBA.
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