Article Figures & Data
Figures
- FIGURE 1.
Biodistribution of 125I-bFGF in 2 groups of experimental animals, Tg(+) and control, given intranasal injection of 125I-bFGF (33.3 MBq/μL per 10 g of body weight). (A) Brain distribution of 125I-bFGF is depicted as mean ± SD (n = 5 in each group). *P < 0.05 vs. control. (B) Whole-body distribution of 125I-bFGF is depicted as mean ± SEM (n = 5 in each group). When SEM is not depicted, it was too small to be shown.
- FIGURE 2.
Adjacent serial sections indicate that immunostaining with antisera to AβPP shows localization of amyloid plaques (A) and that intranasally injected SAP binds to amyloid plaques in Tg mouse cortex as detected by antibody to SAP (B). (D) Brain sections from Tg mice receiving bovine serum albumin plus vehicle immunostained for SAP show no reaction in amyloid plaques, although many plaques are seen on adjacent serial sections stained for AβPP (C). Scale bar = 50 μm. Arrows mark same plaques in serial sections (A and B) and (C and D).
- FIGURE 3.
Light microscopic study of bFGF localization in AβPP Tg mouse cortex, with and without intranasal bFGF injection. bFGF is not readily detectable in neurons in Tg(−) mice after receiving bFGF intranasally (A) or in Tg(+) mice without bFGF injection (B). (C) Nasally injected bFGF bound to cytoplasm of neurons around Aβ deposits in frontal, parietal, and occipital regions in Tg(+) mice. Scale bar = 50 μm.
- FIGURE 4.
Electron microscopy immunogold staining in neuritic plaques in Tg(+) mouse cortex. (A) Tg(+) mice with intravenous injection of bFGF do not show presence of bFGF in brain (original magnification, ×8,000). (B) Same area as in A under higher magnification (×20,000). (C) Representative electron micrograph shows that peripheral margins of neuritic plaques from animals that received bFGF intranasally contain bFGF immunogold reactivity (original magnification, ×50,000). (D) bFGF is also associated with amyloid fibrils in plaque after intranasal injection (original magnification, ×50,000). Arrows indicate bFGF gold labeling. Scale bars = 10 nm.
