Effects of Methylene Blue Stabilizer on In Vitro Viability and Chemotaxis of 99mTc-Exametazime-Labeled Leukocytes

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TABLE 1

Effects of Methylene Blue Stabilizer on Performance of Leukocytes

Labeling formulation	Chemotactic index			Labeling efficiency at 0 h (%)	In vitro stability at 3 h (%)
Labeling formulation		1 h	3 h	Labeling efficiency at 0 h (%)	In vitro stability at 3 h (%)
Control	2.4 ± 0.1	2.4 ± 0.2	NA	NA
a	2.5 ± 0.3	2.2 ± 0.2	85.8 ± 6.3	95.7 ± 1.0
b	2.9 ± 0.9	2.5 ± 0.1	83.8 ± 6.1	94.6 ± 3.0
c	2.5 ± 0.3	2.6 ± 0.5	87.0 ± 6.0	94.7 ± 1.3

Control = leukocytes not labeled with nonstabilized or stabilized ^99mTc-exametazime; NA = not applicable; a = leukocytes labeled with nonstabilized ^99mTc-exametazime; b = leukocytes labeled with stabilized ^99mTc-exametazime containing 250 μg methylene blue; c = leukocytes labeled with stabilized ^99mTc-exametazime containing 500 μg methylene blue.

Data are expressed as mean ± SD (n = 6).