Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI

User menu

  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Nuclear Medicine
  • SNMMI
    • JNM
    • JNMT
    • SNMMI Journals
    • SNMMI
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Journal of Nuclear Medicine

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Past Issues
    • JNM Supplement
    • SNMMI Annual Meeting Abstracts
    • Continuing Education
    • JNM Podcasts
  • Subscriptions
    • Subscribers
    • Institutional and Non-member
    • Rates
    • Journal Claims
    • Corporate & Special Sales
  • Authors
    • Submit to JNM
    • Information for Authors
    • Assignment of Copyright
    • AQARA requirements
  • Info
    • Reviewers
    • Permissions
    • Advertisers
  • About
    • About Us
    • Editorial Board
    • Contact Information
  • More
    • Alerts
    • Feedback
    • Help
    • SNMMI Journals
  • View or Listen to JNM Podcast
  • Visit JNM on Facebook
  • Join JNM on LinkedIn
  • Follow JNM on Twitter
  • Subscribe to our RSS feeds
Meeting ReportMolecular Targeting Probes - Radioactive & Nonradioactive

A Cy5.5-labeled dimeric NGR peptide for near-infrared fluorescence imaging of CD13 receptor expression

Guoquan Li, Yan Xing, Jing Wang, Peter Conti and Kai Chen
Journal of Nuclear Medicine May 2014, 55 (supplement 1) 114;
Guoquan Li
1Department of Radiology, University of Southern California, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yan Xing
1Department of Radiology, University of Southern California, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jing Wang
2Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi’an, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Conti
1Department of Radiology, University of Southern California, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kai Chen
1Department of Radiology, University of Southern California, Los Angeles, CA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
Loading

Abstract

114

Objectives In this study, we synthesized a novel Cy5.5-labeled dimeric NGR peptide (Cy5.5-NGR2) via bioorthogonal click chemistry, and evaluated the utility of Cy5.5-NGR2 for near-infrared fluorescence (NIRF) imaging of CD13 receptor expression in vivo.

Methods The dimeric NGR peptide (NGR2) was conjugated with an alkyne-containing PEG unit followed by mixing with an azide-terminated Cy5.5 fluorophore (Cy5.5-N3) to afford Cy5.5-NGR2. The probe was subject to in vitro and in vivo evaluations. Each mouse bearing an HT-1080 tumor was intravenously injected with 1.5 nmol of Cy5.5-NGR2 and imaged at various time points with or without 20 mg/kg of unlabeled NGR peptide. The animals from non-blocking and blocking groups were euthanized at 4 h pi. The tumors, tissues, and organs were dissected for ex vivo fluorescence imaging.

Results The bioorthogonal click chemistry provided a rapid conjugation of the alkyne-containing NGR2 with Cy5.5-N3 in a quantitative yield within 15 min. The laser confocal microscopy revealed that binding of Cy5.5-NGR2 to CD13 receptor is target-specific as demonstrated in CD13-positive HT-1080 cells, CD13-negative MCF-7 cells, and a blocking study in HT-1080 cells. For In vivo optical imaging, Cy5.5-NGR2 exhibited rapid HT-1080 tumor targeting at 0.5 h pi, and highest tumor-to-background contrast at 2 h pi. The CD13-specific tumor accumulation of Cy5.5-NGR2 was accomplished by a blocking study with unlabeled NGR peptide in HT-1080 tumor bearing mice. The tumor-to-muscle ratio of Cy5.5-NGR2 at 2 h pi reached 2.65±0.13 in non-blocking group vs. 1.05±0.06 in blocking group. The results from ex vivo imaging were consistent with the in vivo findings.

Conclusions We have successfully constructed a Cy5.5-labeled dimeric NGR peptide, which is a promising molecular probe, not only allowing the NIR optical imaging of CD13 overexpressed tumors, but also facilitating noninvasive monitoring of CD13-targeted tumor therapy.

Research Support This work was supported by the USC Department of Radiology, the Major Program of National Natural Science Foundation of China (Grant No. 81230033), the National Basic Research and Development Program of China (Grant No. 2011CB707704), and the Major Research Instrumentation Program of National Natural Science Foundation of China (Grant No. 81227901).

Previous
Back to top

In this issue

Journal of Nuclear Medicine
Vol. 55, Issue supplement 1
May 2014
  • Table of Contents
  • Index by author
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Journal of Nuclear Medicine.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A Cy5.5-labeled dimeric NGR peptide for near-infrared fluorescence imaging of CD13 receptor expression
(Your Name) has sent you a message from Journal of Nuclear Medicine
(Your Name) thought you would like to see the Journal of Nuclear Medicine web site.
Citation Tools
A Cy5.5-labeled dimeric NGR peptide for near-infrared fluorescence imaging of CD13 receptor expression
Guoquan Li, Yan Xing, Jing Wang, Peter Conti, Kai Chen
Journal of Nuclear Medicine May 2014, 55 (supplement 1) 114;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
A Cy5.5-labeled dimeric NGR peptide for near-infrared fluorescence imaging of CD13 receptor expression
Guoquan Li, Yan Xing, Jing Wang, Peter Conti, Kai Chen
Journal of Nuclear Medicine May 2014, 55 (supplement 1) 114;
Twitter logo Facebook logo LinkedIn logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
  • Info & Metrics

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

Molecular Targeting Probes - Radioactive & Nonradioactive

  • Synthesis and Preliminary Evaluation of Metabolically Stable 18F-Lableled PET Tracer for Fatty Acid Beta-Oxidation Imaging.
  • A Unique 18F-labeled G-protein-coupled receptor 44 (GPR44) radiotracer: design, radio-synthesis and evaluation in the rodents
  • 18F-Radiofluorination of Aryl C-H Bonds using a Tandem Ir C-H Borylation/Cu Radiofluorination Strategy
Show more Molecular Targeting Probes - Radioactive & Nonradioactive

Novel Nonradioactive Probes

  • VPAC1 targeted detection of genitourinary cancer: a urinary assay
  • Near-infrared fluorescence imaging with PSVue-T-643 for monitoring phosphatidylserine-targeted cell death induced by chemotherapy.
  • Curcumin analogues as fluorescence imaging probes for brown adipose tissue and monitoring browning
Show more Novel Nonradioactive Probes

Similar Articles

SNMMI

© 2025 SNMMI

Powered by HighWire