Abstract
We assessed the efficiency and toxicity of brief intraperitoneal radioimmunotherapy using high activities of 125I-labeled monoclonal antibody (mAb) in the treatment of small-volume peritoneal carcinomatosis. Methods: Brief intraperitoneal radioimmunotherapy consisted of a 185-MBq (740 MBq/mg) intraperitoneal injection of 125I-35A7 (an anti–carcinoembryonic antigen mAb) into athymic nude mice 4 d after peritoneal tumor xenografting and, after 1 h, abundant washing of the peritoneal cavity with saline solution to remove unbound radioactivity. Another group of mice received this treatment plus a 37-MBq intravenous injection of 125I-35A7 on day 7 or 11 after grafting. Control groups received a brief treatment followed by an additional intravenous injection on day 7 of either saline solution or irrelevant 125I-PX. Tumor growth was monitored by bioluminescence imaging and SPECT/CT, and hematologic toxicity was evaluated by complete blood counts. Survival time was reported, and the mice were sacrificed when the bioluminescence signal reached 4.5 × 107 photons/s. The biodistribution of 125I-35A7 mAb after intravenous or brief treatment was assessed, and the mean absorbed irradiation dose by organs and tumors was calculated using the MIRD formalism. Results: Mild, transient hematologic toxicity was observed after the brief treatment plus intravenous 125I-mAb, with no weight loss. Median survival increased from 32 d in the control groups, to 46 d in the brief treatment group, to 66 d in the group additionally receiving intravenous treatment on day 11, to 73 d in the group additionally receiving intravenous treatment on day 7. The brief treatment alone resulted in a 3-fold higher tumor-to-blood uptake ratio than did the standard intravenous treatment, and the mean absorbed irradiation doses by tumors were 11.6 Gy for the brief treatment and 16.7 Gy for the additional intravenous treatment. For healthy tissues other than blood, the mean absorbed irradiation dose did not exceed 1 Gy after brief treatment and 4.2 Gy after intravenous treatment. Conclusion: The efficiency, low toxicity, and high tumor–to–healthy tissue uptake ratio associated with brief intraperitoneal 125I-35A7 radioimmunotherapy suggest that this method can be used in combination with radiation-synergistic drugs in the therapy of small-volume peritoneal carcinomatosis after cytoreductive surgery.
Peritoneal carcinomatosis is a common evolution of gastrointestinal or gynecologic tumors, or of primary peritoneal malignancies such as mesothelioma or peritoneal serous carcinoma. Peritoneal carcinomatosis has long been considered a terminal disease, having a median survival of 12–23 mo in patients with stage IV ovarian cancer, about 6 mo in colorectal carcinoma patients, 3 mo in gastric cancer patients, 2 mo in pancreatic cancer patients, and only 1.5 mo in patients with carcinomatosis from an unknown primary cancer (1,2). The therapeutic approach is based on palliative systemic chemotherapy and surgery, according to symptoms, except in the case of ovarian cancer, for which complete cytoreduction is part of the standard therapeutic regimen. Twenty years ago, Sugarbaker introduced the use of cytoreductive surgery to resect the visible disease combined with hyperthermic intraperitoneal (IP) chemotherapy to treat the residual disease, as an innovative option for selected patients with peritoneal carcinomatosis (3,4). The procedure for cytoreductive surgery depends on the extent of the peritoneal disease, and the chemotherapy protocols may include mitomycin C, oxaliplatin, mitoxantrone, cisplatin, and irinotecan alone or in combination (1,2). Hyperthermic IP chemotherapy can be performed using open- or closed-abdomen techniques, and perfusion may vary from 30 to 90 min. Although the consensus about the ideal technique is not clear, cytoreductive surgery with hyperthermic IP chemotherapy has been shown to improve the survival of patients with peritoneal dissemination from peritoneal pseudomyxoma, colorectal cancer, and diffuse peritoneal mesothelioma (1,2,5,6). Mortality is relatively low, but morbidity does occur, from surgical complications or from toxicity (such as leukopenia; anemia; thrombopenia; and heart, liver, or kidney toxicity) due to the cytostatic agents (1,2).
Several studies on rodents have shown that radioimmunotherapy (RIT) is an efficient adjuvant after cytoreductive surgery for peritoneal carcinomatosis (7–9). Several IP RIT studies using strong β- or α-emitters are ongoing in animals (8–13). So far, 5 antibodies (against MUC1, CA-125, TAG-72, and gp38) have been conjugated to 4 β-emitting radionuclides for IP RIT in patients with ovarian cancer (14). Based on previous encouraging results (15–19), a phase III randomized multicenter study was undertaken (20) in which the efficiency of conventional chemotherapy was compared with IP injection of 90Y-labeled HMGF1 murine mAb (anti-MUC1). However, no improvement in survival was observed after RIT, although peritoneal recurrence was significantly delayed. One explanation was that the irradiation dose delivered to the tumors was not high enough and that IP injection alone did not target all tumor deposits. Indeed, the induced toxicity by β- and γ-emitters is a drawback for a treatment scheme using repeated injections. One alternative could be the use of short-range particles such as α-emitters (21) or Auger electron emitters. We previously showed that anti–carcinoembryonic antigen (CEA) mAbs labeled with Auger electron emitters and intravenously injected into mice xenografted with cancer cells could significantly delay the growth of small peritoneal solid tumors (7,23). We thus wanted to assess the efficiency and toxicity of 125I-mAb when used in IP RIT for mice bearing small peritoneal tumors. In addition, because the effects of IP RIT performed after hyperthermic IP chemotherapy have never, to our knowledge, been investigated, we decided to perform RIT using a closed peritoneal perfusion of 125I-labeled anti-CEA mAb for a short period (1 h), followed by extensive washing of the peritoneal cavity with NaCl (saline) solution to remove the unbound radioactivity. We call this procedure brief IP RIT.
MATERIALS AND METHODS
Cell Line and mAb
The vulvar squamous carcinoma cell line A-431 expressing the epidermal growth factor receptor and transfected as previously described (22), with constructs encoding the CEA and luciferase genes, was used. The cells were grown as previously described (22). The noninternalizing murine IgG1k 35A7 mAb, specific for the CEA Gold 2 epitope, was used to target CEA. The irrelevant PX antibody was used for control experiments. PX is an IgG1 mAb that has been purified from the mouse myeloma MOPC 21 (24). PX and 35A7 were purified from mouse hybridoma ascitic fluids by ammonium sulfate precipitation followed by ion exchange chromatography on DE52 cellulose (Whatman).
Radiolabeling
125I was from Perkin Elmer, and mAbs were radiolabeled at the specific activity of 740 MBq/mg for RIT and biodistribution studies, using the IODO-GEN (Pierce Chemical Co.) method and previously described methods (22). The immunoreactivity of 125I-mAb against CEA was assessed in vitro by direct binding assay (22). The binding percentage was determined by measuring the antigen-bound radioactivity after 2 washes with phosphate-buffered saline and was between 50% and 60%.
Animals
Athymic nude mice (female, 6–8 wk old) were obtained from Charles River and were acclimated for 1 wk before experimental use. They were housed at 22°C and 55% humidity with a light–dark cycle of 12 h. Food and water were available ad libitum. The mice were force-fed with Lugol solution the day before RIT, and stable iodine was added to drinking water for the entire experimental period. Body weight was determined weekly, and the mice were clinically examined throughout the study. Hematologic toxicity was monitored for 70 d after the start of RIT, using the scil Vet abc system (SCIL Animal Care Co.). All animal experiments were performed in compliance with the guidelines of the French government and the standards of Institut National de la Santé et de la Recherche Médicale for experimental animal studies (agreement B34-172-27).
RIT Experiments
Mice were intraperitoneally grafted with 0.7 × 106 A-431 cells suspended in 0.3 mL of Dulbecco modified Eagle medium. Tumor growth was assessed 3 d after cell xenografting by bioluminescence imaging, which allowed the segregation of mice into homogeneous groups. Four days after grafting, the mice underwent IP or brief IP RIT.
The protocol for brief IP RIT was as follows. After being anesthetized by IP injection of a solution containing ketamine (100 mg/kg [Ketamine Panpharma; Panpharma]) and medetomidine (1 mg/kg [Dormitor; Pfizer]), the mice were intraperitoneally injected with either saline or 125I-mAb in a final volume of 5 mL. One hour later, the peritoneal cavity was flushed with 25 mL of saline for 15 min, using a perfusion system adapted from Aarts et al. (8). Typically, an inflow needle was placed in the upper abdominal cavity and 2 multiperforated catheters were inserted laterally through the abdominal wall to be used as outflows (Fig. 1A). Perfusion was done using a peristaltic pump (Harvard Apparatus). Once the peritoneal cavity had been washed, the catheters were removed and the mice weighed. They were then awakened by an IP injection of atipamezole (2.5 mg/kg of body weight [Antisedan; Pfizer]).
The protocol for IP RIT consisted of a standard IP injection of either saline or 125I-mAb (final volume of 5 mL) without washing of the peritoneal cavity, and intravenous (IV) RIT on day 7 or 11 was done conventionally, as previously described (22).
For brief IP RIT, one group of mice (n = 8) was treated with 5 mL of saline and another (n = 10) with 185 MBq of 125I-35A7 mAb. For IP RIT, the control group (n = 7) received a 5-mL IP injection of saline, whereas the other group (n = 15) received one 37-MBq IP injection of 125I-35A7 mAb on day 4 after grafting and another on day 7. For the combined brief IP and IV RIT, mice received brief IP 125I-35A7 RIT on day 4 after grafting and an IV 37-MBq injection of 125I-35A7 on day 7 or 11 (n = 9). To assess the nonspecific efficiency of 125I-mAb, another group was treated with brief IP RIT using 185 MBq of 125I-PX mAb followed by IV injection of 37 MBq of 125I-PX mAb on day 7.
Tumor growth was followed weekly by bioluminescence imaging. Mice were sacrificed when the bioluminescence signal reached a value of 4.5 × 107 photons/s, corresponding to a total tumor weight of about 0.2–0.3 g.
Bioluminescence and SPECT/CT
In vivo bioluminescence imaging was performed after IP injection of luciferin (0.1 mg of luciferin/g) as previously described (22). Whole-body SPECT/CT images were acquired at various times after brief IP 125I-35A7 RIT (0, 1, 24, and 72 h) with a 2-head multiplexing multipinhole NanoSPECT camera (Bioscan Inc.).
Biodistribution Experiments to Mimic Brief IP RIT and IV RIT
On day 1, 48 athymic nude mice were intraperitoneally grafted with 0.7 × 106 A-431 cells that had been suspended in 0.3 mL of Dulbecco modified Eagle medium. Mice were divided into 2 groups to compare the biodistribution of 125I-35A7 mAb after brief IP RIT or IV RIT. To test IP biodistribution, one group of mice was treated with brief IP 125I-35A7 RIT according to the previously described protocol, but the injected solution contained 5.5 MBq (740 MBq/mg) of 125I-35A7 mAb completed with 243 μg of unlabeled 35A7 mAb diluted in 5 mL.
To test IV biodistribution, a second group was intravenously injected with a solution containing 185 kBq (740 MBq/mg) of 125I-35A7 mAb completed with 50 μg of unlabeled 35A7 mAb diluted in 300 μL of saline solution.
The mice were sacrificed at 1, 24, 48, 72, 96, 144, and 168 h after treatment. At each time point, animals were anesthetized; bioluminescence images acquired; and the animals euthanized, bled, and dissected. Blood and healthy organs were weighed. The size of tumor nodules was determined, the tumor volume calculated, and thereby the tumor weight calculated on the basis of a density of 1.05 g/cm3 as described by Santoro et al. (22). The radioactivity uptake during the biodistribution experiments was then measured for tumor nodules and for all organs, using a γ-well counter. The percentage of injected activity per gram of tissue, corrected for radioactive decay, was calculated for IV 125I-35A7 biodistribution. For brief IP 125I-35A7 biodistribution, results were expressed as percentage of the remaining activity per gram of tissue immediately after the peritoneal wash (i.e., 1 h after injection).
Tumor Weight Assessment During RIT Experiments
Accurate, direct weighing of peritoneal tumors was not possible in RIT experiments because of the high activities and because the mice would have to be sacrificed; therefore, weights were calculated from the weekly bioluminescence imaging data. For this purpose, biodistribution experiments were used to determine the calibration curve between the bioluminescence signal of tumors and their size. Typically, before sacrifice, tumors were imaged by bioluminescence and the corresponding signal (photon/s) was correlated with the calculated tumor weight (g) determined by direct measurement of the size of tumor nodules, as described previously (22). The calibration curve was similar to that determined in our previous work (22).
Uptake of Radioactivity per Organ and Tumor and Dosimetry
The uptake of radioactivity per tissue (Bq) in RIT experiments was extrapolated from the uptake per tissue measured during the biodistribution experiments. Because the activities used in RIT experiments were 33 times higher than those used in the biodistribution analysis (185 MBq vs. 5.5 MBq) for the same amount of injected mAb (250 μg), all uptake values from the biodistribution experiments were multiplied by 33 to mimic therapeutic conditions. We considered that the weight of healthy tissues did not change during the study and that they did not differ between RIT and biodistribution experimental conditions. We confirmed that this assumption was true also for tumor nodules during the first week after injection. Therefore, the 33-fold correction was enough to distinguish uptake of radioactivity during the RIT experiments from uptake of radioactivity during the biodistribution experiments. The total cumulative decays per tissue, Ãrs, were calculated by measuring the area under the curves for the uptake of radioactivity during the RIT experiments. Following the MIRD formalism (25), the resulting values were multiplied by the S factor for determining the irradiation doses as determined in our previous work (22).
Statistical Analysis
Kaplan–Meier survival estimates were calculated from the date of the xenograft until the date of the event of interest (i.e., a bioluminescence value of 4.5 × 107 photons/s) and compared with the Log-rank test. Statistical analysis was performed using STATA 10.0 software.
RESULTS
Tumor Growth
On day 4 after grafting (just before treatment began), 5–6 nodules per mouse with a mean diameter of about 1.5–2 mm were detected by bioluminescence imaging. This size corresponded to a mean tumor weight of 1.2 ± 0.9 × 10−2 g. In the brief IP saline group, tumors grew exponentially and all mice were sacrificed before day 40 after grafting (Fig. 1B). A similar growth rate was observed in the group treated by brief IP + IV day 7 125I-PX RIT (data not shown). The longest delay in tumor growth was observed in the group treated by brief IP + IV day 7 125I-35A7 RIT. Intermediary tumor growth kinetics were reported for the groups treated by brief IP 125I-35A7 RIT (Fig.1B), brief IP + IV day 11 125I-35A7 RIT, and IP + IP day 7 125I-35A7 RIT (data not shown).
Washing of the peritoneal cavity with saline slowed tumor growth in the brief IP saline group, compared with the IP saline group (data not shown). This observation was further strengthened by the analysis of survival data; survival was significantly lower after IP saline (P < 0.001, compare Fig. 2A with Fig. 2B).
Toxicity of Brief IP RIT Using 125I-mAb
The residual activity per mouse was about 14.2 ± 7.3 MBq immediately after the wash with saline solution and dropped to 2.1 ± 0.7 MBq at 120 h after brief IP RIT (Fig. 1C). These results indicate that about 7.6% of the injected activity was effectively kept within the peritoneal cavity. No weight loss was observed after brief IP RIT (Supplemental Fig. 1). These results suggest that brief IP RIT is well tolerated by mice. However mild, transient hematologic toxicity was reported in all treated mice, in comparison to the control brief IP saline group (Fig. 3 and Supplemental Fig. 2). In the brief IP 125I-35A7 RIT group (Fig. 3), the nadir for white blood cells was reached between days 7 and 10 (around −20%) after grafting and was due mainly to a decrease in lymphocyte and monocyte levels (−40%) (Supplemental Fig. 2). The lowest platelet point occurred slightly later (days 10–15, −30%), whereas no modification in red blood cells was observed. Most values returned to normal around day 39. In mice that received an IV injection of 125I-mAb on day 7 (brief IP + IV day 7 125I-35A7 RIT and brief IP + IV day 7 125I-PX RIT), the decrease in white blood cells was more pronounced and prolonged (about −70% on day 22; Fig. 3). When the IV injection was done on day 11 (brief IP + IV day 11 125I-35A7 RIT) after grafting, the hematologic toxicity was in the same range but more prolonged, and values had not yet returned to baseline by day 39 (Fig. 3 and Supplemental Fig. 2). The ratio for day 52 could not be calculated, since most of the mice in the brief IP saline group had to be sacrificed because of tumor growth.
The similar effects observed for mice receiving brief IP + IV day 7 125I-35A7 RIT and brief IP + IV day 7 125I-PX RIT suggest that the hematologic toxicity was mainly due to nonspecific irradiation, including soft x-rays or the most energetic electrons emitted by 125I. In addition, IP + IP day 7 125I-35A7 RIT (Fig. 3 and Supplemental Fig. 2) caused lower hematologic toxicity than did brief IP + IV day 7 125I-35A7 RIT, despite the higher activities detected in IP + IP day 7 125I-35A7 RIT mice. Indeed, during the latter IP RIT, 74 MBq (2 × 37 MBq) were effectively injected and available for diffusion toward the blood compartment. By contrast, for brief IP + IV RIT, of the 185 MBq used, only 14.2 MBq were remaining in the mouse immediately after washing of the peritoneal cavity. This activity was completed by IV injection of 37 MBq; that is, a final total activity of 51.2 MBq was effectively remaining in the mouse. This result suggests that the high activity of 185 MBq maintained for 1 h during brief IP RIT is mainly responsible for the hematologic toxicity.
Improved Survival with Brief IP 125I-35A7 RIT
Mice were sacrificed when the bioluminescence signal reached 4.5 × 107 photons/s, corresponding to a mean tumor weight of about 0.2–0.3 g. The median survival was about 32 d in the brief IP saline group but significantly increased (P < 0.001) to 46 d in the group treated with brief IP 125I-35A7 RIT (Fig. 2A). This value was significantly improved (P < 0.001) when brief IP RIT was combined with an IV injection on day 11 (brief IP + IV day 11 125I-35A7 RIT; median survival = 66 d) or day 7 (brief IP + IV day 7 125I-35A7 RIT; median survival = 73 d) (Fig. 2A). Moreover, 1 mouse in each of these 2 groups was cured by the end of the observation period of 100 d. Mice treated by brief IP + IV day 7 125I-PX RIT had a median survival of 31 d—somewhat similar to the control brief IP saline group (P = 0.188) (Fig. 2A), suggesting the absence of toxicity and the efficiency of 125I when unbound to cells. Finally, median survival was 49 d in the IP + IP day 7 125I-35A7 RIT mice (Fig. 2B) and only 23 d in the IP + IP day 7 saline RIT control group (P < 0.001). Moreover one of the IP + IP day 7 125I-35A7 RIT mice was cured by the end of the period of interest.
SPECT/CT After Brief IP 125I-35A7 RIT
Whole-body SPECT/CT showed that after injection, radioactivity was homogeneously distributed in the peritoneal cavity (Fig. 4A). After washing of the peritoneal cavity, the radioactivity concentrated at tumor nodules up to 3 d after injection (Fig. 4A), as indicated by the good fitting with the bioluminescence signals (Fig. 4B).
Improved Tumor-to-Healthy Tissues Uptake Ratio with Brief IP 125I-35A7 RIT
The biodistribution study confirmed the strong uptake of 125I-35A7 mAb by tumor nodules (Fig. 5A). The percentage of remaining activity per gram of tumor immediately after washing of the peritoneal cavity ranged from 72.1% ± 30.2% at 1 h to 20.5% ± 4.8% at 168 h. These values were much higher than the peak value of 27.8% ± 7.2% of injected activity per gram of tumor determined at 96 h after a single IV injection of 37 MBq (740 MBq/mg), typical of IV 125I-35A7 RIT (Fig. 5B). Moreover, the uptake of radioactivity by healthy organs was higher after IV injection than after brief IP 125I-35A7 RIT. For instance, in brief IP 125I-35A7 biodistribution mice, the peak value in blood at 48 h was 12.2% ± 3.2% of remaining activity per gram, whereas this peak value was about 28.1% ± 2.4% of injected activity per gram in the IV 125I-35A7 biodistribution group at 1 h after injection (Fig. 5B). These results suggest that brief IP 125I-35A7 RIT improves tumor targeting and that healthy tissues are more protected, compared with IV injection.
Cumulated Uptake of Radioactivity After Brief IP 125I-35A7 RIT and Dosimetry
From the biodistribution data, we expressed the uptake of radioactivity of 125I-35A7 mAb by healthy organs and tumor nodules as a function of time (Figs. 6A and 6B and Supplemental Fig. 3).
The cumulated uptake of radioactivity (Ãrs) was then determined by calculating the area under the curves shown in Figures 6A and 6B together with Supplemental Figure 3. According to the MIRD formalism, the mean absorbed irradiation dose per organ was calculated by multiplying Ãrs by the S value corresponding to 125I. The mean absorbed irradiation dose by tumors was 11.6 Gy after brief IP 125I-35A7 RIT and 16.7 Gy after IV 125I-35A7 RIT. The irradiation dose delivered to healthy organs after brief IP 125I-35A7 RIT was much lower than that after IV 125I-35A7 RIT: for instance, blood received about 1.9 Gy after brief IP 125I-35A7 RIT and about 9.8 Gy after IV 125I-35A7 RIT (Fig. 6C). The irradiation dose to the other organs did not exceed 1 Gy after brief IP 125I-35A7 RIT and 4.2 Gy after IV 125I-35A7 RIT.
DISCUSSION
In the present study, we investigated the feasibility, toxicity, and efficacy of brief IP RIT using a 125I-mAb directed against CEA. This protocol is based on the hyperthermic IP chemotherapy methodology, although hyperthermia was not associated with brief IP RIT. Our results indicate that about 7% of the injected activity, corresponding to about 14.2 MBq, was retained in the peritoneal cavity after washing. Mild and transient blood toxicity was observed and was mainly due to nonspecific irradiation (possibly soft x-rays and more energetic electrons emitted by 125I) that occurred during the 1-h peritoneal incubation with high activities of 125I-mAb. However, we estimated that in patients, about 300 μGy would be delivered by such an external irradiation at the sacral level (data not shown). Therefore, the hematologic toxicity is expected to be much lower than in mice.
SPECT/CT of mice at different time points after brief IP 125I-35A7 RIT, and the biodistribution experiments, indicate that radioactivity was concentrated in tumor nodules, whereas the irradiation dose to healthy organs by brief IP 125I-35A7 RIT was generally much lower than with IV 125I-35A7 RIT and did not exceed 1 Gy. In addition, although the irradiation dose delivered to the tumors was lower after brief IP 125I-35A7 RIT than after IV 125I-35A7 RIT, the tumor-to-blood irradiation dose ratio was about 5 for brief IP 125I-35A7 RIT and 1.7 for IV 125I-35A7 RIT. These results indicate that brief IP 125I-35A7 RIT protects healthy tissues while delivering significant irradiation doses to tumors, confirming the generally described advantage of IP over IV RIT for peritoneal disease in terms of concentration and tolerance (14). Therefore, although reducing the potential blood reservoir of 125I-mAb in comparison to IV RIT, the brief IP RIT procedure, which includes washing of the peritoneal cavity, eliminates undesirable radioactivity and therefore is less toxic.
The median survival of xenografted mice was significantly improved after brief IP 125I-35A7 RIT alone (46 d) in comparison to controls (32 d). However, the combination of brief IP 125I-35A7 RIT on day 4 and IV 125I-35A7 RIT on day 7 further increased survival to 73 d. In our previous study (22), median survival was 59 d (vs. 19 d for controls) for mice treated with 2 IV injections of 37 MBq of 125I-35A7 mAb. Assuming that the transient hematologic toxicity we observed after brief IP 125I-35A7 RIT is effectively due to the initial 1-h incubation with 125I-mAb in the peritoneal cavity, repeated IV injections of 125I-mAb could be planned after brief IP RIT—or the combination of brief IP 125I-35A7 RIT and chemotherapy might be envisaged—without a significant increase of toxicity, as we observed in the brief IP + IV day 7 125I-35A7 RIT group. The median survival increased to 49 d also after 2 IP injections of anti-CEA mAb (IP + IP day 7 125I-35A7 RIT), with low associated hematologic toxicity. However, the effect on survival was weaker than the improvement obtained after brief IP + IV day 7 125I-35A7 RIT (73 vs. 49 d; whereas controls had a median survival of 32 and 23 d, respectively). Moreover, the uptake of radioactivity by healthy tissues during brief IP RIT was low and was probably much lower than during IP RIT. In addition, the combination of brief IP RIT and IV RIT takes advantage of the better peritoneal tumor uptake obtained through the IP route, and the delayed IV injection may allow reaching pockets of cancer cells that were not targeted by brief IP RIT (26).
Completeness of resection and tumor load are the most important predictors of long-term survival after cytoreductive surgery and hyperthermic IP chemotherapy. In the present study, we targeted tumor nodules of about 1.2 ± 0.9 × 10−2 g. It was shown with β-emitters that RIT must be dedicated to small solid tumors (27). The size parameter is even more crucial when low-energy electron emitters are used, because the path length of electrons emitted by 125I ranges from nanometers to about 20 μm, whereas in peritoneal carcinomatosis, tumor nodules have a diameter of several millimeters. Our results can be compared with the work of Aarts et al., who found that when RIT was combined with cytoreductive surgery, median survival increased from 57 to 97 d in rats that had CC-531 colon carcinoma tumor xenografts of a few millimeters and were treated with a single 74-MBq IP injection of 177Lu-labeled MG1 mAb (8,9). The low toxicity for healthy tissue of Auger electron emitters such as 125I is expected to be an advantage for the treatment of small peritoneal carcinomatosis, especially because of the potential for combining RIT with radiation-synergistic chemotherapy, such as taxol (28,29), or drugs targeting the tumor microenvironment (30–32). In addition, repeated 125I-mAb injections will also be made possible. The precise mechanisms by which 125I-anti CEA mAb efficiently delays tumor growth remain to be elucidated. Considering that most of the electron dose generated by 125I decay is extremely localized, most of the irradiation dose must be delivered to the cell membrane when targeted by 125I-anti-CEA mAb. Therefore, we can hypothesize that the cell membrane is an important target of high-LET Auger electrons.
CONCLUSION
Our data confirm the efficiency of 125I-anti CEA mAb in killing tumor cells. Brief IP RIT is accompanied by a strong tumor–to–healthy tissue ratio that allows an increase in injected activities while protecting healthy tissues. This is reinforced by the use of Auger electron emitters such as 125I, as they decrease nonspecific irradiation. All these features might allow the use of repeated injections of radiolabeled antibodies or the combination of RIT with radiation-synergistic agents. Therefore, our results suggest that brief IP RIT with 125I-labeled anti-CEA mAb might be a complementary tool in the therapy of small solid tumors after cytoreductive surgery.
Acknowledgments
This work was supported by the Institut National du Cancer, grant R09080FF/RPT09005FFA. We thank Imade Ait Arsa for animal care and involvement in the experiments.
- © 2010 by Society of Nuclear Medicine
REFERENCES
- Received for publication June 15, 2010.
- Accepted for publication August 27, 2010.