[PDF][PDF] Technetium-99m labeling and biodistribution of anti-TAC disulfide-stabilized Fv fragment

TM Yoo, HK Chang, CW Choi, KO Webber… - Journal of Nuclear …, 1997 - Soc Nuclear Med
MATERIALS AND METhODS Radiolabeling The production of anti-Tac dsFv has been
described (1). Anti Tac dsFv used for this study recognizes the alpha subunit of the IL-2 …

Lowering of pI by acylation improves the renal uptake of 99mTc-labeled anti-Tac dsFv: effect of different acylating reagents

I Kim, H Kobayashi, TM Yoo, M Kim, N Le, E Han… - Nuclear medicine and …, 2002 - Elsevier
Anti-Tac disulfide-stabilized variable region fragment (dsFv) was labeled with 99mTc by a
preformed chelate approach using 99mTc-MAG3-trifluorophenyl (TFP) ester. Simultaneously …

Similarities in the biodistribution of iodine-labeled anti-Tac single-chain disulfide-stabilized Fv fragment and anti-Tac disulfide-stabilized Fv fragment

H Kobayashi, ES Han, IS Kim, N Le… - Nuclear medicine and …, 1998 - Elsevier
We evaluated the biodistribution and pharmacokinetics of two different iodine-labeled Fv
fragments of anti-Tac monoclonal antibody (MAb) in normal and tumor-bearing nude mice …

Aminosyn II Effectively Blocks Renal Uptake of 18F-labeled Anti-Tac Disulfide-stabilized Fv

JA Carrasquillo, L Lang, M Whatley, P Herscovitch… - Cancer research, 1998 - AACR
Because intact IgG has limitations as a tumor-imaging agent, radiolabeled Fv fragments are
being evaluated. Due to the high renal accumulation of Fv fragments, methods to block renal …

L-Lysine Effectively Blocks Renal Uptake of 125I- or 99mTc-labeled Anti-Tac Disulfide-stabilized Fv Fragment

H Kobayashi, TM Yoo, IS Kim, MK Kim, N Le… - Cancer research, 1996 - AACR
In this study, we investigated the ability of L-lysine to block renal uptake of 125I-or 99mTc-
labeled Fv fragments. Anti-Tac disulfide-stabilized Fv fragment (dsFv) was derived from a …

Chemical Modification to Reduce Renal Uptake of Disulfide-Bonded Variable Region Fragment of Anti-Tac Monoclonal Antibody Labeled with 99mTc

IS Kim, TM Yoo, H Kobayashi, M Kim, N Le… - Bioconjugate …, 1999 - ACS Publications
The anti-Tac disulfide-bonded variable region fragment (dsFv) is a genetically engineered,
25 kDa, murine monoclonal antibody fragment that recognizes the α subunit of the …

Rapid and specific uptake of anti-Tac disulfide-stabilized Fv by interleukin-2 receptor-bearing tumors

KO Webber, RJ Kreitman, I Pastan - Cancer research, 1995 - AACR
The disulfide-stabilized Fv (dsFv) is a novel form of a variable-region fragment (Fv) of an
antibody which is stabilized by an interchain disulfide bond. As a consequence, it is more …

Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc (I)–carbonyl complex

R Waibel, R Alberto, J Willuda, R Finnern… - Nature …, 1999 - nature.com
We have developed a technetium labeling technology based on a new organometallic
chemistry, which involves simple mixing of the novel reagent, a 99m Tc (I)–carbonyl …

Hexa-histidine tag as a novel alternative for one-step direct labelling of a single-chain Fv antibody fragment with 99mTc

GJ Pimentel, JE Vazquez, W Quesada… - Nuclear medicine …, 2001 - journals.lww.com
From genetic material of hybridoma cells, we have generated a recombinant single-chain
antibody fragment (scFv antibody) specific to carcinoembryonic antigen (CEA), which can …

Improved Biodistribution of 125I-labeled Anti-Tac Disulfide-stabilized Fv Fragment by Blocking Its Binding to the α Subunit of the Interleukin 2 Receptor in the …

H Kobayashi, TM Yoo, D Drumm, MK Kim, BF Sun… - Cancer research, 1997 - AACR
Animal studies using radiolabeled anti-Tac disulfide-stabilized Fv (dsFv) monoclonal
antibody have shown formation of complexes in serum with the soluble α subunit of the …