Avidin* and Streptavidin* were both conjugated with DTPA using the cyclic anhydride of
DPTA*(13). The proteins were prepared at a concentration of 20 mg/ml in 0.05 M …

This report describes preliminary results in a limited clinical trial of the pretargeting concept
in patients in which streptavidin and biotin have been employed be cause of the high affinity …

SUMMARY 1.[2'-14C] Biotin has been synthesized from 14CO in 70% radiochemical yield.
2. A rapid and simple assay for avidin has been worked out, based on the determination of …

MATERIALS AND METHODS Attachment of DTPA to proteins. DTPA was at tached to HSA
according to the method of Krejca rek and Tucker (5). DTPA (100 mg) and triethylam inc …

Several monoclonal antibodies (MAbs), reactive with tumor‐associated antigens, selectively
persist on tumor sites in vivo for many days. If biotinylated, such highly specific tags on tumor …

As biotin-avidin systems continue to be developed for applications involving single cells, cell
suspensions, and especially tissue sections, the need arises for a method of blocking …

The covalent attachment of strong chelating groups such as DTPA to IgG antibodies may
simplify the labeling of these proteins with 99mTc and may improve the stability of the label …

Abstract Labeling of F (ab′) 2 with 99m Tc was investigated. The best labeling procedure
for F (ab′) 2 was then applied to IgG and Fab. Stannous ion was used as the reducing …

In recent years, the avidin-biotin complex has become very useful as an extemely versatile,
general mediator in a wide variety of bioanalytical applications. The rapid rise in its appeal …

A commercially available, purified preparation of avidin was found to comprise two
polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as …