The pET series of expression plasmids are widely used for recombinant protein production
in Escherichia coli. The genetic modules controlling transcription and translation in these …

Membrane proteins can be examined in near‐native lipid‐bilayer environments with the
advent of polymer‐encapsulated nanodiscs. These nanodiscs self‐assemble directly from …

Here we describe the cryo-electron microscopy structure of the human histamine 2 receptor (H
2 R) in an active conformation with bound histamine and in complex with G s …

Cell-free expression enables direct cotranslational insertion of G protein coupled receptors (GPCRs)
and other membrane proteins into the defined membrane environments of …

Nanoparticles composed of amphiphilic scaffold proteins and small lipid bilayers are
valuable tools for reconstitution and subsequent functional and structural characterization of …

123 I-metaiodobenzylguanidine ( 123 I-MIBG) has independent prognostic value for risk
stratification among heart failure patients, but the use of concomitant medication should not …

Cell-free protein expression systems and lipid nanoparticle technologies are core platforms
for membrane protein synthesis. The implementation of preassembled nanodiscs allows the …

Bacteria are widely employed as cell factories to produce recombinant proteins that are used
as biopharmaceuticals and industrial enzymes. Typically, a protein coding sequences is …

This fully updated edition targets not only those assays directly involved in the discovery of
GPCR-active compounds but also those involved in cell-based experiments designed to …