Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins

VN Kokryakov, SSL Harwig, EA Panyutich… - FEBS …, 1993 - Wiley Online Library
Porcine leukocytes contained three homologous peptides, PG‐1, 2 and 3, that manifested
potent microbicidal activity against Escherichia coli, Listeria monocytogenes and Candida …

Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes

SSL Harwig, KM Swiderek, VN Kokryakov, L Tan… - FEBS letters, 1994 - Elsevier
We purified three homologous antimicrobial peptides (‘gallinacins’) from chicken leukocytes,
examined their antimicrobial activity in vitro, and established their primary sequences by a …

[PDF][PDF] Solution structure of protegrin-1, a broad-spectrum antimicrobial peptide from porcine leukocytes

RL Fahrner, T Dieckmann, SSL Harwig, RI Lehrer… - Chemistry & biology, 1996 - cell.com
Background: The protegrins are a family of arginine- and cysteine-rich cationic peptides
found in porcine leukocytes that exhibit a broad range of antimicrobial and antiviral activities. …

Clavanins, α‐helical antimicrobial peptides from tunicate hemocytes

IH Lee, C Zhao, Y Cho, SSL Harwig, EL Cooper… - Febs …, 1997 - Wiley Online Library
Hemocytes from the invertebrate Styela clava, a solitary tunicate, contained a family of four α‐helical
antimicrobial peptides that were purified, sequenced and named clavanins A, B, C …

Intramolecular disulfide bonds enhance the antimicrobial and lytic activities of protegrins at physiological sodium chloride concentrations

SSL Harwig, A Waring, HJ Yang, Y Cho… - European journal of …, 1996 - Wiley Online Library
Protegrins are 2‐kDa antimicrobial peptides that contain 16–18 amino acid residues and two
intramolecular disulfide bonds. We studied the contribution of these disulfide bonds to the …

Prophenin-1, an exceptionally proline-rich antimicrobial peptide from porcine leukocytes

SSL Harwig, VN Kokryakov, KM Swiderek… - FEBS letters, 1995 - Elsevier
We purified and characterized an unusual antimicrobial peptide, prophenin-1 (PF-1), from
porcine leukocytes. The peptide had a mass of 8,683 and contained 79 residues, including 42 …

Neutrophil defensins: purification, characterization, and antimicrobial testing

SSL Harwig, T Ganz, RI Lehrer - Methods in enzymology, 1994 - Elsevier
Publisher Summary This chapter describes the large-scale purification of the four defensins,
HNP-1, -2, -3, and -4, found in human neutrophils and describes procedures for testing their …

[HTML][HTML] Characterization of defensin precursors in mature human neutrophils

SSL Harwig, ASK Park, RI Lehrer - Blood, 1992 - Elsevier
Human defensins HNP-1 and -3 are broad spectrum antimicrobial peptides that are synthesized
by human neutrophils as 94 amino acid (aa) precursors that require proteolytic removal …

Determination of disulphide bridges in PG‐2, an antimicrobial peptide from porcine leukocytes

SSL Harwig, KM Swiderek, TD Lee… - Journal of peptide …, 1995 - Wiley Online Library
We determined the cysteine connectivity of protegrin PG‐2, a leukocyte‐derived antimicrobial
peptide, by performing sequential enzyme digestions with chymotrypsin and thermolysin, …

Purification of cysteine-rich bioactive peptides from leukocytes by continuous acid-urea-polyacrylamide gel electrophoresis

SSL Harwig, NP Chen, ASK Park, RI Lehrer - Analytical biochemistry, 1993 - Elsevier
A new continuous acid-urea-polyacrylamide gel electrophoresis (CAU-PAGE) preparative
method was developed and used to purify rabbit and human defensins. With it, we identified …