Advances in molecular biology, organic chemistry, and materials science have recently created
several new classes of fluorescent probes for imaging in cell biology. Here we review the …

Recombinant proteins containing four cysteines at the i,i + 1, i + 4, and i + 5 positions of an α
helix were fluorescently labeled in living cells by extracellular administration of 4′,5′-bis(1…

Green fluorescent proteins (GFPs) are presently attracting tremendous interest as the first
general method to create strong visible fluorescence by purely molecular biological means. So …

… The intercept of the Perrin plot of FlAsH−Cys 6,7,10,11 -calmodulin indicates a limiting
anisotropy r 0 of 0.35. More significantly, the linearity of the points irrespective of temperature …

Recombinant proteins containing tetracysteine tags can be successively labeled in living
cells with different colors of biarsenical fluorophores so that older and younger protein …

FLUORESCENCEimaging is perhaps the most powerful technique currently available for
continuously observing the dynamic intracellular biochemistry of single living cells 1 . However, …

" Caged" compounds are artificial molecules whose biological actIvity is controlled by light,
usually by photolytic conversion from an inactive to an active form. The term caged has …

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase, labeled with fluorescein
and rhodamine on the catalytic and regulatory subunits, respectively, was injected into …

Regulation of AMPA receptor (AMPAR) trafficking is important for neural plasticity. Here we
examined the trafficking and synthesis of the GluR1 and GluR2 subunits using ReAsH-EDT 2 …

Fluorescence resonance energy transfer (FRET) from cyan to yellow fluorescent proteins (CFP/YFP)
is a well-established method to monitor protein-protein interactions or …