Isopropanol production with engineered Cupriavidus necator as bioproduction platform

E Grousseau, J Lu, N Gorret, SE Guillouet… - Applied microbiology …, 2014 - Springer
Alleviating our society’s dependence on petroleum-based chemicals has been highly emphasized
due to fossil fuel shortages and increasing greenhouse gas emissions. Isopropanol is …

Metabolic engineering of Cupriavidus necator for heterotrophic and autotrophic alka (e) ne production

L Crépin, E Lombard, SE Guillouet - Metabolic engineering, 2016 - Elsevier
Alkanes of defined carbon chain lengths can serve as alternatives to petroleum-based fuels.
Recently, microbial pathways of alkane biosynthesis have been identified and enabled the …

Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process

S Alfenore, C Molina-Jouve, S Guillouet… - Applied Microbiology …, 2002 - Springer
Several bottlenecks in the alcoholic fermentation process must be overcome to reach a very
high and competitive performance of bioethanol production by the yeast Saccharomyces …

Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production

…, E Lombard, AJ Sinskey, N Gorret, SE Guillouet - Metabolic …, 2017 - Elsevier
We previously reported a metabolic engineering strategy to develop an isopropanol producing
strain of Cupriavidus necator leading to production of 3.4 g L −1 isopropanol. In order to …

Impacts of environmental conditions on product formation and morphology of Yarrowia lipolytica

A Timoumi, SE Guillouet, C Molina-Jouve… - Applied microbiology …, 2018 - Springer
The yeast Yarrowia lipolytica is an industrially important microorganism with distinctive
physiological and metabolic characteristics. A variety of external factors (eg, pH, temperature, and …

Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed‐batch and continuous cultures

…, JL Uribelarrea, N Gorret, SE Guillouet… - Microbial …, 2015 - Wiley Online Library
Formic acid, acting as both carbon and energy source, is a safe alternative to a carbon dioxide,
hydrogen and dioxygen mix for studying the conversion of carbon through the C alvin– B …

D-stat culture for studying the metabolic shifts from oxidative metabolism to lipid accumulation and citric acid production in Yarrowia lipolytica

A Ochoa-Estopier, SE Guillouet - Journal of Biotechnology, 2014 - Elsevier
Lipid accumulation in oleaginous yeasts is triggered by nutrient imbalance in the culture
medium between the carbon source in excess and the nitrogen source in limiting concentration. …

Highly Enantioselective Synthesis of No‐Carrier‐Added 6‐[18F]Fluoro‐L‐dopa by Chiral Phase‐Transfer Alkylation

C Lemaire, S Gillet, S Guillouet… - European Journal of …, 2004 - Wiley Online Library
[ 18 F]Fluoro‐L‐dopa, an important radiopharmaceutical for positron emission tomography (PET),
has been synthesized using a phase‐transfer alkylation reaction. A chiral quaternary …

Minimization of Glycerol Production during the High-Performance Fed-Batch Ethanolic Fermentation Process in Saccharomyces cerevisiae, Using a Metabolic Model …

…, JL Uribelarrea, SE Guillouet - Applied and …, 2006 - Am Soc Microbiol
On the basis of knowledge of the biological role of glycerol in the redox balance of
Saccharomyces cerevisiae, a fermentation strategy was defined to reduce the surplus formation of …

Isopropanol production from carbon dioxide in Cupriavidus necator in a pressurized bioreactor

…, L Maignien, E Lombard, J Singh, SE Guillouet - New biotechnology, 2020 - Elsevier
A bioreactor was designed to provide high gas mass transfer to reach cell and product titres
in the g L -1 level from CO 2 for realistic, laboratory scale, engineered autotrophic strain …