Subclass restriction of murine anti-carbohydrate antibodies

…, D Hansburg, DE Briles, RA Nicolotti… - The Journal of …, 1978 - journals.aai.org
Examination of the subclass distribution of murine antibodies directed against groups A and
C streptococcal carbohydrate, α-(1→3) dextran and phosphocholine yields the surprising …

Immunological properties of model membranes

SC Kinsky, RA Nicolotti - Annual Review of Biochemistry, 1977 - annualreviews.org
CLASSICAL PATHWAY Traditionally, complement (C) is defined as a system of nine serum
protein components that react in a specific order (Cl, C4,. C2, C3, C5, C6, C7, C8, C9) to …

Antibody formation in response to liposomal model membranes sensitized with N-substituted phosphatidylethanolamine derivatives

K Uemura, RA Nicolotti, HR Six, SC Kinsky - Biochemistry, 1974 - ACS Publications
Kei-ichi Uemura, Robert A. Nicolotti, Howard R. Six, and Stephen C. Kinsky* abstract: This
investigation demonstrates that the immuno-genicity of lipids, which is a known property of …

Isoelectric focusing of immunoglobulins: improved methodology

RA Nicolotti, DE Briles, JA Schroer, JM Davie - Journal of Immunological …, 1980 - Elsevier
Conditions for isoelectric focusing and detecting antibodies in thin layers of polyacrylamide
have been evaluated and several improvements have been made. First, we have developed …

Immunogenicity of liposomal model membranes sensitized with mono (p-azobenzenearsonic acid) tyrosylphosphatidylethanolamine derivatives. Antibody formation …

RA Nicolotti, SC Kinsky - Biochemistry, 1975 - ACS Publications
Robert A. Nicolotti and Stephen C. Kinsky* abstract: We have previously reported that
hapten spe-cific antibodies are produced in guinea pigs immunized with certain N-substituted …

Stimulation and inhibition of anti-hapten responses in guinea pigs immunized with hybrid liposomes.

N Kochibe, RA Nicolotti, JM Davie… - Proceedings of the …, 1975 - National Acad Sciences
Guinea pigs were immunized with liposomal model membranes containing
phosphatidylethanolamine (PE) or glycerophosphorylethanolamine (GPE) derivatives in which the …

Development of a model system to evaluate methods for radiolabeling monoclonal antibodies.

…, MJ Welch, KD McElvany, RA Nicolotti… - Journal of Nuclear …, 1984 - europepmc.org
We have developed a model system that can be used to evaluate methods for radiolabeling
monoclonal antibodies. In this system, dinitrophenyl (DNP) is used as a model antigen, and …

Comparative immunogenic properties of N-substituted phosphatidylethanolamine derivatives and liposomal model membranes

RA Nicolotti, N Kochibe, SC Kinsky - The Journal of Immunology, 1976 - journals.aai.org
This study describes some of the parameters that quantitatively or qualitatively influence the
immunogenicity in guinea pigs of synthetic lipid antigens: phosphatidylethanolamine (PE) …

Lipid metabolism during mediator release from mast cells: studies of the role of arachidonic acid metabolism in the control of phospholipid metabolism.

DL Marquardt, RA Nicolotti, DA Kennerly… - Journal of immunology …, 1981 - journals.aai.org
Recent studies indicate that both arachidonic acid (AA) metabolism and phospholipid (PL)
metabolism are markedly stimulated during the release of mediators from mast cells. The …

Radiolabeled antibody fragments

RA Nicolotti - 1989 - osti.gov
This patent describes an antibody conjugate of the formula: wherein Ab is the residue of a
Fab fragment of a monoclonal antibody. The antibody being one which retains antigen-…