Display on the yeast cell wall is well suited for engineering mammalian cell-surface and
secreted proteins (eg, antibodies, receptors, cytokines) that require endoplasmic reticulum-…

… display, K d YSD , and other methods, K d other . scFv binding to fluorescein; K d other
determined by fluorescence quenching. scFv binding botulinum neurotoxin type A; K d other …

A nonimmune library of 10 9 human antibody scFv fragments has been cloned and expressed
on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead …

… Schmidt, K. Dane Wittrup; A modeling analysis of the effects of molecular size and binding
affinity on tumor targeting. Mol Cancer Ther 1 October 2009; 8 (10): 2861–2871. https://doi.org/…

… 2 P R cap R Krogh 2 ( e − k clear t − e − k e t ) k e − k clear where [Ab] tumor is the antibody
… capillary), k clear is the effective first-order systemic clearance rate constant, and k e is the …

Single-chain antibody mutants have been evolved in vitro with antigen-binding equilibrium
dissociation constant K d = 48 fM and slower dissociation kinetics (half-time > 5 days) than …

… Typical scFv k on values are about 1 × 10 5 M −1 s −1 , and k off = K d × k on can be
calculated from the estimated K d of the sample. However, k on can increase substantially during …

Antibodies have proven to be effective agents in cancer imaging and therapy. One of the major
challenges still facing the field is the heterogeneous distribution of these agents in tumors …

Checkpoint blockade with antibodies specific for cytotoxic T lymphocyte–associated protein (CTLA)-4
or programmed cell death 1 (PDCD1; also known as PD-1) elicits durable tumor …

The described protocols enable thorough screening of polypeptide libraries with high
confidence in the isolation of improved clones. It should be emphasized that the protocols have …