Chloramine-T in high-specific-activity radioiodination of antibodies using N-succinimidyl-3-(trimethylstannyl)benzoate as an intermediate

Nucl Med Biol. 1998 Oct;25(7):659-65. doi: 10.1016/s0969-8051(98)00033-x.

Abstract

Monoclonal antibodies C215 and MOv18 have been radiohalogenated, using a single-batch method employing N-succinimidyl-3-(trimethylstannyl)benzoate, m-MeATE. Labelling to the stannyl ester was optimized using chloramine-T as oxidizing agent. The results show that the stannyl ester is effectively labelled with short reaction times giving reproducible yields from 85% to 95%. Subsequent antibody conjugation, 10 to 80 microg MAb, resulted in biologically active, labelled antibodies with overall radiochemical yields of 50% to 80%, with corresponding specific activities of 490-50 kBq(125I)/microg.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / chemistry*
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / metabolism
  • Antibody Affinity
  • Benzoates / chemistry*
  • Chloramines / chemistry*
  • Chromatography, High Pressure Liquid
  • Iodine Radioisotopes / chemistry*
  • Organotin Compounds / chemistry
  • Radiopharmaceuticals / chemical synthesis
  • Tosyl Compounds / chemistry*
  • Trimethyltin Compounds / chemistry*
  • Tumor Cells, Cultured / immunology

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Benzoates
  • Chloramines
  • Iodine Radioisotopes
  • Organotin Compounds
  • Radiopharmaceuticals
  • Tosyl Compounds
  • Trimethyltin Compounds
  • chloramine-T
  • N-succinimidyl 3-(trimethylstannyl)benzoate