Abstract
Monoclonal antibodies C215 and MOv18 have been radiohalogenated, using a single-batch method employing N-succinimidyl-3-(trimethylstannyl)benzoate, m-MeATE. Labelling to the stannyl ester was optimized using chloramine-T as oxidizing agent. The results show that the stannyl ester is effectively labelled with short reaction times giving reproducible yields from 85% to 95%. Subsequent antibody conjugation, 10 to 80 microg MAb, resulted in biologically active, labelled antibodies with overall radiochemical yields of 50% to 80%, with corresponding specific activities of 490-50 kBq(125I)/microg.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / chemistry*
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / metabolism
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Antibody Affinity
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Benzoates / chemistry*
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Chloramines / chemistry*
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Chromatography, High Pressure Liquid
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Iodine Radioisotopes / chemistry*
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Organotin Compounds / chemistry
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Radiopharmaceuticals / chemical synthesis
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Tosyl Compounds / chemistry*
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Trimethyltin Compounds / chemistry*
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Tumor Cells, Cultured / immunology
Substances
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Antibodies
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Antibodies, Monoclonal
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Benzoates
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Chloramines
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Iodine Radioisotopes
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Organotin Compounds
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Radiopharmaceuticals
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Tosyl Compounds
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Trimethyltin Compounds
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chloramine-T
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N-succinimidyl 3-(trimethylstannyl)benzoate