Metabolic and transcriptional changes in osteosarcoma cells treated with chemotherapeutic drugs

U Haberkorn; F Oberdorfer; T Klenner; L G Strauss; M Stöhr; R Wallich; A Altmann; G V Kaick

doi:10.1016/0969-8051(94)90163-5

Metabolic and transcriptional changes in osteosarcoma cells treated with chemotherapeutic drugs

Nucl Med Biol. 1994 Aug;21(6):835-45. doi: 10.1016/0969-8051(94)90163-5.

Authors

U Haberkorn¹, F Oberdorfer, T Klenner, L G Strauss, M Stöhr, R Wallich, A Altmann, G V Kaick

Affiliation

¹ Department of Oncologic Diagnosis and Therapy, German Cancer Research Center, Heidelberg, Germany.

PMID: 9234333
DOI: 10.1016/0969-8051(94)90163-5

Abstract

Two cell lines derived from a lung metastasis of a rat osteosarcoma were treated with cisplatin (CDDP) and two phosphonic acid compounds (AMDP, DADP), AMDP-treated cells showed a decrease in FDG uptake, CDDP and DADP resulted in an increase. A block in G2 or in S and G2 phase was seen after CDDP and AMDP treatment. The changes in the cell cycle fractions were not related to the changes in FDG uptake. Furthermore, the transcription of the glucose transporter and hexokinase genes were elevated in CDDP and decreased in AMDP treated cells. However, the changes in FDG uptake were not fully explained by changes at the transcriptional level. The total uptake of thymidine was elevated although the incorporation of thymidine into DNA decreased. In both cell lines the changes in FDG uptake correlated with the changes in thymidine incorporation into DNA (r = 0.95 and r = 0.83, respectively). Cells with an increased FDG uptake showed a weaker growth inhibition than cells with a decrease in FDG uptake.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use*
Bone Neoplasms / drug therapy*
Bone Neoplasms / genetics
Bone Neoplasms / metabolism
Cisplatin / pharmacology
Cisplatin / therapeutic use*
Deoxyglucose / analogs & derivatives
Deoxyglucose / metabolism
Flow Cytometry
Fluorodeoxyglucose F18
Lung Neoplasms / genetics
Lung Neoplasms / metabolism*
Lung Neoplasms / secondary
Organophosphorus Compounds / pharmacology
Organophosphorus Compounds / therapeutic use
Organoplatinum Compounds / pharmacology
Organoplatinum Compounds / therapeutic use
Osteosarcoma / drug therapy*
Osteosarcoma / genetics
Osteosarcoma / metabolism
Rats
Thymidine / metabolism
Transcription, Genetic / drug effects*

Substances

Antineoplastic Agents
Organophosphorus Compounds
Organoplatinum Compounds
Fluorodeoxyglucose F18
aminotris(methylenephosphonato)diamminoplatinum (II)
diamminecyclohexanoaminotrismethylenephosphonatoplatinum(II)
Deoxyglucose
Cisplatin
Thymidine