Abstract
High effective specific activity N-succinimidyl 4-[18F](fluoromethyl)benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (< 100 micrograms) of sensitive proteins such as erythropoietin can be labeled with this reagent in high yield without aggregation.
MeSH terms
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / isolation & purification
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Benzoates / chemical synthesis
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Benzoates / chemistry*
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Benzoates / isolation & purification
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Chromatography, High Pressure Liquid
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Erythropoietin / chemistry
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Erythropoietin / isolation & purification
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Fluorine Radioisotopes
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Indicators and Reagents
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Proteins / chemistry*
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Proteins / isolation & purification
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Succinimides / chemical synthesis
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Succinimides / chemistry*
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Succinimides / isolation & purification
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Transferrin / chemistry
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Transferrin / isolation & purification
Substances
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Antibodies, Monoclonal
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Benzoates
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Fluorine Radioisotopes
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Indicators and Reagents
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N-succinimidyl 4-(fluoromethyl)benzoate
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Proteins
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Succinimides
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Transferrin
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Erythropoietin