Pretargeted tumour imaging with streptavidin immunoconjugates of monoclonal antibody CC49 and 111In-DTPA-biocytin

Nucl Med Biol. 1996 May;23(4):459-66. doi: 10.1016/0969-8051(96)00022-4.

Abstract

Pretargeted tumour imaging was performed in nude mice bearing subcutaneous LS174T human colon cancer xenografts with streptavidin-CC49 monoclonal antibody and 111In-DTPA-biocytin. Mice were administered 250 micrograms of streptavidin-CC49, followed 6 or 9 days later by 40 ng (250 microCi) of 111In-DTPA-biocytin. Tumors were visualized at 24 h postinjection of 111In-DTPA-biocytin. Tumour uptake was 0.9-2.5% injected dose/g with tumour/nontarget ratios from 2:1 to 37:1 (except for kidney, which was 0.5-3:1). Tumour uptake in mice pretargeted with streptavidin or streptavidin-conjugated nonspecific normal mouse IgG was < 0.1% i.d./g.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / pharmacokinetics
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • Bacterial Proteins* / pharmacokinetics
  • Colonic Neoplasms / diagnostic imaging*
  • Colonic Neoplasms / metabolism
  • Female
  • Glycoproteins / immunology
  • Glycoproteins / metabolism
  • Humans
  • Immunoconjugates* / pharmacokinetics
  • Indium Radioisotopes* / pharmacokinetics
  • Lysine / analogs & derivatives*
  • Lysine / pharmacokinetics
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Pentetic Acid / analogs & derivatives*
  • Pentetic Acid / pharmacokinetics
  • Radionuclide Imaging
  • Streptavidin
  • Tissue Distribution
  • Transplantation, Heterologous

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Bacterial Proteins
  • Glycoproteins
  • Immunoconjugates
  • Indium Radioisotopes
  • alpha,omega-(bis)DTPA-biocytin
  • tumor-associated antigen 72
  • Pentetic Acid
  • Streptavidin
  • Lysine