Abstract
A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteriophage lambda / genetics*
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Cell Line
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Cell Survival*
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Chlorocebus aethiops
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Cloning, Molecular
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DNA, Viral / administration & dosage
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DNA, Viral / metabolism*
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Drug Carriers
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Gene Transfer Techniques*
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Genes, Bacterial
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Glutamates
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Liposomes
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Pentosyltransferases / biosynthesis*
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Pentosyltransferases / genetics
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Phosphatidylcholines
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Phosphatidylethanolamines
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Plasmids*
Substances
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DNA, Viral
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Drug Carriers
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Glutamates
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Liposomes
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Phosphatidylcholines
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Phosphatidylethanolamines
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didodecyl N-(alpha-trimethylammonioacetyl)glutamate
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1,2-dilauroylphosphatidylcholine
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1,2-dielaidoylphosphatidylethanolamine
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Pentosyltransferases
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xanthine phosphoribosyltransferase