Detection of thymidine kinase activity using an assay based on the precipitation of nucleoside monophosphates with lanthanum chloride

R M Wolcott; J M Colacino

doi:10.1016/0003-2697(89)90352-7

Detection of thymidine kinase activity using an assay based on the precipitation of nucleoside monophosphates with lanthanum chloride

Anal Biochem. 1989 Apr;178(1):38-40. doi: 10.1016/0003-2697(89)90352-7.

Authors

R M Wolcott¹, J M Colacino

Affiliation

¹ Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.

PMID: 2729577
DOI: 10.1016/0003-2697(89)90352-7

Abstract

A new assay method for the measurement of thymidine kinase (TK) is described. Cytosols were prepared from TK- and TK+ cells and evaluated for TK activity using an assay which is based on the phosphorylation of [125I]-iododeoxyuridine, [125I]-iododeoxycytidine, or [3H]thymidine and the precipitation of the monophosphates of these nucleosides by lanthanum chloride. The specificity, reproducibility, sensitivity, and convenience of this assay are demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bromodeoxycytidine / analogs & derivatives
Cells, Cultured
Chemical Precipitation
Cytosol / enzymology
Deoxycytidine / analogs & derivatives
Deoxycytidine / metabolism
Idoxuridine / metabolism
Lanthanum / metabolism*
Nucleotides / metabolism*
Thymidine / metabolism
Thymidine Kinase / analysis*

Substances

Nucleotides
lanthanum chloride
Deoxycytidine
Bromodeoxycytidine
ibacitabine
Lanthanum
Thymidine Kinase
Idoxuridine
Thymidine