mFISH analysis of irradiated human fibroblasts: a comparison among radiations with different quality in the low-dose range

Radiat Prot Dosimetry. 2015 Sep;166(1-4):302-5. doi: 10.1093/rpd/ncv189. Epub 2015 Apr 20.

Abstract

The present investigation aimed to characterise the shape of dose-response curve and determining the frequency distribution of various aberration types as a function of dose and radiation quality in AG01522 primary human fibroblasts in the 0.1- to 1-Gy dose range. For this purpose, the cells were irradiated with 7.7 and 28.5 keV µm(-1) low-energy protons, 62 keV µm(-1 4)He(2+) ions (LNL Radiobiology facility) or X rays and samples collected for 24-colour mFISH analysis. X rays and 7.7 keV µm(-1) protons displayed a quadratic dose-response curve solely for total and simple exchanges, whereas for high-linear energy transfer radiations, a linear dose-response curve was observed for all the aberration categories, with the exception of complex exchanges.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Aberrations / radiation effects*
  • Fibroblasts / radiation effects*
  • Heavy Ions*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Linear Energy Transfer / radiation effects*
  • Protons*
  • Radiation Dosage
  • X-Rays

Substances

  • Protons