Test-retest variability of adenosine A2A binding in the human brain with (11)C-TMSX and PET

Mika Naganawa; Masahiro Mishina; Muneyuki Sakata; Keiichi Oda; Mikio Hiura; Kenji Ishii; Kiichi Ishiwata

doi:10.1186/s13550-014-0076-9

Test-retest variability of adenosine A2A binding in the human brain with (11)C-TMSX and PET

EJNMMI Res. 2014 Dec 29;4(1):76. doi: 10.1186/s13550-014-0076-9. eCollection 2014 Dec.

Authors

Mika Naganawa¹, Masahiro Mishina², Muneyuki Sakata³, Keiichi Oda⁴, Mikio Hiura⁵, Kenji Ishii³, Kiichi Ishiwata³

Affiliations

¹ PET Center, Yale University School of Medicine, 801 Howard Avenue, PO Box 208048, New Haven, CT 06520-8048 USA ; Research Team for Neuroimaging, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015 Japan.
² Research Team for Neuroimaging, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015 Japan ; Department of Neurological Science, Graduate School of Medicine, Nippon Medical School, Tokyo, 113-0022 Japan.
³ Research Team for Neuroimaging, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015 Japan.
⁴ Department of Radiological Technology, Faculty of Health Sciences, Hokkaido University of Science, Hokkaido, 006-8585 Japan.
⁵ Faculty of Sports and Health Studies, Hosei University, Tokyo, 194-0298 Japan.

Abstract

Background: The goal of the present study was to evaluate the reproducibility of cerebral adenosine A2A receptor (A2AR) quantification using (11)C-TMSX and PET in a test-retest study.

Methods: Five healthy volunteers were studied twice. The test-retest variability was assessed for distribution volume (V T) and binding potential relative to non-displaceable uptake (BPND) based on either metabolite-corrected arterial blood sampling or a reference region. The cerebral cortex and centrum semiovale were used as candidate reference regions.

Results: Test-retest variability of V T was good in all regions (6% to 13%). In the putamen, BPND using the centrum semiovale displayed a lower test-retest variability (3%) than that of BPND using the cerebral cortex as a reference region (5%). The noninvasive method showed a higher or similar level of test-retest reproducibility compared to the invasive method.

Conclusions: Binding reproducibility is sufficient to use (11)C-TMSX as a tool to measure the change in A2AR in the human brain.

Keywords: 11C-TMSX; Adenosine A2A receptor; Positron emission tomography; Reproducibility.