In vivo scintigraphic imaging of proteoglycans

Elisabeth Miot-Noirault; Aurélien Vidal; Philippe Auzeloux; Caroline Peyrode; Jean-Claude Madelmont; Jean-Michel Chezal

doi:10.1007/978-1-61779-498-8_13

In vivo scintigraphic imaging of proteoglycans

Methods Mol Biol. 2012:836:183-98. doi: 10.1007/978-1-61779-498-8_13.

Authors

Elisabeth Miot-Noirault¹, Aurélien Vidal, Philippe Auzeloux, Caroline Peyrode, Jean-Claude Madelmont, Jean-Michel Chezal

Affiliation

¹ INSERM, UMR 990, Clermont-Ferrand, France.

PMID: 22252636
DOI: 10.1007/978-1-61779-498-8_13

Abstract

In this chapter, we present the methods developed in our lab for the scintigraphic imaging and direct quantitative evaluation of proteoglycan (PG) distribution in vivo. These methods relate to (1) the synthesis and radiolabeling of the NTP 15-5 with (99m)Tc, (2) preclinical scintigraphic imaging using laboratory animals, and (3) quantitative analysis of scintigraphic images.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Heterocyclic Compounds, 1-Ring / chemistry
Mice
Proteoglycans / analysis*
Quaternary Ammonium Compounds / chemistry
Rabbits
Radionuclide Imaging*
Rats
Technetium / chemistry

Substances

Heterocyclic Compounds, 1-Ring
N-(triethylammonium)-3-propyl(15)ane-N5
Proteoglycans
Quaternary Ammonium Compounds
Technetium