Abstract
We examined the production, separation, and characterization of (89)Zr, including supplementation of a commercial Cyclone(®) 18/9 with a self-made Solid Target System (STS). Obtained [(89)Zr]Zr-oxalate was used for the labeling of anti-B cell antibodies with desferrioxamine-p-SCN as a bifunctional chelator. (89)Zr-labeled antibodies were injected in DBA/1 mice to examine usability for detection of B cells in vivo. PET measurements showed binding of (89)Zr-labeled anti-B cell antibodies in tissues with high frequencies of B cells, i.e. in spleen and lymph nodes.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Anti-Idiotypic / chemistry
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Antibodies, Monoclonal / analysis*
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Antibodies, Monoclonal / chemistry
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B-Lymphocytes / diagnostic imaging*
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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Chelating Agents / chemistry
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Deferoxamine / chemistry
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Indoles / chemistry
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Isotope Labeling
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Lymph Nodes / cytology
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Lymph Nodes / diagnostic imaging
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Mice
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Mice, Inbred DBA
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Mice, Nude
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Positron-Emission Tomography / methods
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Quinoxalines / chemistry
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Radiography
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Radioimmunodetection / methods*
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Radioisotopes / analysis*
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Radioisotopes / chemistry
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Spectrometry, Gamma / methods*
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Spleen / cytology
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Spleen / diagnostic imaging
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Tissue Distribution
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Zirconium / analysis*
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Zirconium / chemistry
Substances
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Antibodies, Anti-Idiotypic
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Antibodies, Monoclonal
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Chelating Agents
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Indoles
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Quinoxalines
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Radioisotopes
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B 220
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Zirconium
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Deferoxamine