The proteome of the human breast cancer cell line MDA-MB-231: Analysis by LTQ-Orbitrap mass spectrometry

Vaïk Strande; Ludovic Canelle; Christophe Tastet; Odile Burlet-Schiltz; Bernard Monsarrat; Hubert Hondermarck

doi:10.1002/prca.200800083

The proteome of the human breast cancer cell line MDA-MB-231: Analysis by LTQ-Orbitrap mass spectrometry

Proteomics Clin Appl. 2009 Jan;3(1):41-50. doi: 10.1002/prca.200800083. Epub 2008 Dec 9.

Authors

Vaïk Strande¹, Ludovic Canelle, Christophe Tastet, Odile Burlet-Schiltz, Bernard Monsarrat, Hubert Hondermarck

Affiliation

¹ INSERM U 908 (JE-2488) "Signalisation des facteurs de croissance dans le cancer du sein. Protéomique fonctionnelle", Institut National de la Santé et de la Recherche Médicale and Université Lille 1, Lille, France.

PMID: 21136935
DOI: 10.1002/prca.200800083

Abstract

We have used a combination of SDS-PAGE and LTQ-Orbitrap MS to explore the proteome of the highly invasive MDA-MB-231 breast cancer cell line. Based on about 520 000 MS/MS spectra, a total of 3481 proteins were identified and subsequently classified according to their cellular distribution and molecular function. Interestingly, a large proportion of proteins (38%) were from cellular membranes and we were able to characterize numerous proteins involved in cancer initiation and progression such as the tumor suppressor p53 and the epidermal growth factor receptor. Together, this study represents the largest proteome database of breast cancer cells realized to date and demonstrates the value of using Orbitrap MS for deeper proteome analysis.